植物病原原核微生物快速检测新技术的应用现状

徐平东; 柯冲

植物病原原核微生物快速检测新技术的应用现状

徐平东¹,
柯冲²

1.
厦门华侨亚热带植物引种园厦门 361002;
2.
福建省农业科学院福州 350003

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出版历程
- 收稿日期: 1990-09-28
- 刊出日期: 1991-05-15

The Recent Advances in New Techniques for A Rapid and Specific Ditection for phytopathogenic Prokaryotes

Xu Pingdong¹,
Ke Chong²

1.
Fruit Research Institute, FuJian Academy of Agricultural Sciences, Fuzhou 350013

摘要

摘要: 本文讨论了近年来发展起来的特异性强、灵敏度高、快速的植物病原原核微生物检测新技术的原理及应用现状。即酶联免疫吸附测定、单克隆抗体、免疫荧光、免疫电镜和核酸杂交等技术的应用进展。
- 植物病原原核微生物 /
- 酶联免疫吸附测定 /
- 单克隆抗体 /
- 免疫荧光 /
- 免疫电镜 /
- 核酸杂交
Abstract: Discussion is made on the recent advances in new techniques for a rapid, sensitive and specific detection for phytopathogenic prokaryotes. Enzyme-linked immunosorbent assay (ELISA), monoclonal antibodies, immunoflurescence, immunosorbent electron microscopy (ISEM) and nucleic acid hybridization are described in relation to phytopathogenic prokaryote diagnosis.
- Phytopathogenic prokaryotes /
- ELISA /
- Monoclonal antibodes /
- immunofluorescence /
- ISEM /
- Nucleic acid hybridization

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参考文献(9)

[3] 韩国安等.1990.用单克隆抗体检测枣疯病类菌原体.南京农业大学学报,13(1):123

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[12] Davis, M.J.,J. H. Tsai,R. L.Cox, L.L.McDaniel,and N. A. Harrison. 1987. DNA probes for detecting the maize-bushy-stunt mycoplasmalike organism(MBS-MLO).(Abstr.) Phytopathology 77:1769

[16] Davis, R. E.,I.M.Lee,S.M. Douglas, and E. L.Dally.1988.Biotinvlated cloned DNA fragments from an unknown mycoplasmalike organism(MLO) defect infection in several plant diseases.(Abstr.)Phytopatology 78:1541

[19] Derrick, K.S.and H. H, Brlansky.1976.Assay for viruses and mycoplasmas using serologically specific electron microscopy. Phytopatology 66:815-820

[30] Kohler, G. and L.M ilstein.1975.O'ontinuous culture of fused cells secreting antibody of predefined specificity. Nature 256:495-497

[42] Milne, R. G, and D. E. Lesemann,1984.Immunosorbent electron microscopy in plant virus studies,In:Methods in Virology Maramorosch、K,and H. Koprowski,eds,)Vol.8:85-101.Academic Press, New York

[50] Stanbridge, E. J. and M.E. Reff. 1979.The molecular biology of mycoplasma. In:The Mycoplasms(Barile, M.F. and S. Razin, eds.)Vol.1:157-187 Academic Press, New York

[51] Symons, R. H. 1984.Diagnostic approaches for the rapid and specific detection of plant viruses and viroids. In:Plant Microbe Interactions; 1 Molecular and Genetic Perspectives.(Kosuge, T. and E. W.Nester, eds.)PP. 93-124.Mac-Millan,New York

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