水稻细菌性条斑病菌单克隆抗体的制备及特性
PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST XANTHOMONAS CAMPESTRIS PV. O.RYZICLA
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摘要: 用7株水稻细菌性条斑病菌(Xanthomonas campestris pv.oryzicola)分别免疫BALB/C小白鼠。建立了15株分泌抗水稻细菌性条斑病菌特异性抗体的杂交瘤细胞株。杂交瘤细胞株的细胞染色体数范围为98—104。酶联免疫吸附试验(ELISA)培养液中的抗体滴度为102—103,腹水中抗体滴度105—106。这些单克隆抗体(McAb)的免疫球蛋白亚类分别属于IgG2a、IgG2b、IgG3和IgM。McAb与水稻细菌性条斑病菌发生特异性反应;与水稻白叶枯病菌(X.c.pv.oryzea)等黄单胞杆菌属的其它变种菌株无交叉反应;与植物病原细菌其它4个属的测定菌株无交叉反应;与稻叶、稻种上分离的杂菌也无交叉反应。Abstract: Eifteen hybridoma cell lines secreting monoclonal antibodies (McAbs) against Xanthomonas campestris pv. oryzicola were established. The chromosome numbers of the hybridoma cell lines ranged from 98 to 104. The ELISA titers of the culture supernatants and the ascitic fluid were 102—103 and 105—106, respectively. Seven of the fifteen McAbs belonged to IgG3, three to IgG2a, one to IgG2b and four to IgM. The reaction of the mcAbs with X. c. pv. oryzicola was specific and none of the McAbs reacted with other patnogenic varieties in X. campestris, such as X. c. pv. oryzae, strains of other 4 plant pathogenic bacterial genera and some becteria on the surface of rice leaves and seeds.
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Key words:
- Monoclonal antibodies /
- Xanthomonas campestris pv. oryzicola /
- production
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