萍藻重组研究Ⅱ、应用孢子果技术获得萍藻重组成功
THE RECOMBINATION BETWEEN ANABAENA-FREE AZOLLA WITH ANABAENA AZOLLAE Ⅱ.THE ACHIEVEMENT OF RECOMBINATIONS BETWEEN ANABAENA-FREE AZOLLA AND ANABAENA AZOLLAE BY USING SPOROCARP TECHNIQUE
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摘要: 应用去除藻体细胞的老熟或萌动的大孢子果直接加入同种或异种红萍大孢子果的鱼腥藻,取得了重新组合,其成功率达10%。重新组合的萍藻,第一步经显微镜观察、固氮酶活性测定,结果表明,重新组合的大孢子果萌发出来的红萍体内具有鱼腥藻;第二步采用单克隆抗体检测验证,确认为萍藻重组成功,其组合为细绿无萍藻×小叶萍鱼腥藻、细绿无藻萍×墨西哥萍鱼腥藻、小叶无藻萍×细绿萍鱼腥藻、细绿无藻萍×细绿萍鱼腥藻。小叶无藻萍×小叶萍鱼腥藻。Abstract: The recombination were achieved by directly putting anabaena from megasporocarp of same or different Azolla species into the ma-tureor germinating megasporocarp which had been eliminated anabaena cells and the recombination rate is 10%. The recombined Azolla was firstly examined under microscope and tested the nitrogenase activity analysis as showing that Anabaena azollae existed in recombined Azolla frond which developed from megasorocarp previously eliminated anabaena cells.Secondly, the analysis by monoclonal antibody also proved the success in recombination and confirmed the newreestablished parnters are: Anabaena-free A. filiculoides譇nabaena azollae from A.microphylla; Anabaena-free A. filiculoides譇nabaena azollae from A. mexiconaj and Anabaeza-free A.microphyllaes譇nabaena azollae from A.Filiculoides and Anabaeza-free A. filiculoides譇nabaena azollae from A filicujo-idesj Anabaena-free A.microphylla譇nabaena azollae from A. microhylla respectively.
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[4] 刘中柱等,1986。红萍鱼腥藻单克隆抗体的研究。福建省农科院学报,1(2):1-7 [7] 郑德英、陈扬春,1980e茎尖培养获得无藻萍。福建农业科技(4),31-32 [10] 廖嘉信等,1979。甘薯无病毒苗培育及病毒检定。中华农业研究,28(3):139-144 -
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