摘要:
利用单克隆抗体,建立了快速诊断新城疫的免疫组化方法,检测病鸡病理组织中的新城疫病毒抗原,结果表明脾、肺、肾和盲肠扁桃体等病科的免疫萤光阳性率分别为86～91%,91～93%,100%和86%。证明这些组织是较理想的送检样本。与病毒分离率(87.2%)比较,免疫萤光的阳性率较高(92.3%),二种方法的符合率为89.7%。综上所述,免疫组化方法是一种简便、快速、准确的方法。单克隆抗体作为诊断试剂具有其独到的优越性。
Abstract:
Immunohistochemistry methods using monoclonal antibodies (McAb) to diagnose Newcastle Disease (ND) have been established.The NDV antigen were found in the organs and tissues of the chickens infected NDV by using these methods. The FA positive rates of the spleen, lung, kidney, and caecum of the cnickens with ND were 86~91%, 91~93%, 100%, 86% respectively, which proved these organs were ideal tested samples for detecting NDV.In a comparison, the positive rate of FA(92.3%) was higher than the rate of virus isolation (87.2%), The concordant rate of the two nctiods was 89.7%.The results showed that the immunohistochemistry methods were simple,rapid and accurate,and that the McAb as a diagnosis reagent was excellent.
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