摘要:
本文用酶细胞化学技术,设置四种酶反应液处理甘蔗茎不同节间的辅导组织,研究对二个甘蔗品种不同节间韧皮部细胞ATPase活性的影响。并观察了甘蔗茎韧皮部细胞膜结合ATP酶活性在不同生长期的变化。结果表明,不同离子和不同pH值对甘蔗茎韧皮部细胞ATP酶活性影响明显不同。Mg++和Cl-对韧皮部ATP酶激活力最大,介质pH6.5时韧皮部细胞ATP酶活性较pH7.6时强。不同生长期,相同节位,韧皮部细胞的ATP酶活性亦有所不同,与甘蔗成熟期糖分积累的增加相符合,表明韧皮部运输的糖分在茎中卸出是靠ATP酶洪能的主动过程;并认为用细胞化学方法,有助于定性地了解难于分离的韧皮部细胞膜结合的ATP酶的特性及其在糖分运输积累上的作用。
Abstract:
The effects of four mediums with different ions and pH value, incubating the transport tissue sections, on ATPase activity of phloem cells of different internodes in two sugarcane varieties was studied in this paper by cytochemical technique. The changes of ATPase activity of phloem cells of sugarcane in different age was also investigated. The results showed that ATPase activity of sugarcane stem phloem has significant difference in the present of different ions and pH value.Mg++ and cl- gave the greatest stimulation to phloem ATPase and the ATPase activity showed higher in the medium with pH 6.5 than with pH 7.2. The phloem ATPase activity of the same position internode in different age showed different, and which consisted with the increase of sugar accumulation with cane ripening. It was suggested that sugar unloaded from phloem in suggarcane stem was active process and speculated that, with the enzyme cytochemical technique, the characteristics of membrane located ATPase activity of phloem cellse which are difficult to isolate and their effection on phloem transport may be known qualitatively and directly.
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