摘要:
本试验采用聚丙烯硫胺凝胶电泳技术,对园艺花叶芋(Caladium hortulanum Birdscy)和双色花叶芋(C. bicolor Vent.)两个种的13个栽培品种的根,进行了酯酶同工酶的测定分析。结果表明,花叶芋有共同的特征性谱带,酶谱依迁移率大小明显可分成慢中快三区,即Ea、Eb和Ec区。品种间谱带的差异集中表现在Ea区,并依其酶带谱型,园艺花叶芋品种可归成三大类群;双色花叶芋的酶谱差异也表现在Ea区,但是多了一条Rf值较大的Ea5带。本文还从生化角度对花叶芋各品种的同工酶酶谱差异与叶片形态的相关性,酶谱不稳定性与形态变异性的关联进行了讨论,从而为阐明花叶芋的种质特征及其园艺分类提供了科学依据。
Abstract:
Caladium is a notable ornamental foliage plant with beautiful and colourful leaves. It is of a tremendous number of horticultural varieties, thus resulting in some confusion of identification and nomenclature.Esterase isozymic patterns of roots of 13 caladium varieties were detected by using the technique of polyacrylamide gel electrophoresis on the vertical slabs.The results of the study showed that the bands observed in gels were grouped, according to their different migrants, into three regions, that is, Ea,Eb and Ec. The zymograms between varieties had no significant differences at the regions Eb and EC. The more anodal region Ea was apparently variable.On the basis of the zymogramatic patterns, all varieties of Caladium nortulanum Birdsey tested may be classified into three groups. C. bicolor Veat was also variable at Ea, where a band of faster mobility(Ea5)appeared.In the light of biochemistry the relationships were discussed between zymogram patterns and blade shapes,between the unstability of zymogram and the variability of morphology, thus providing scientific basis for expounding the characteristic features and classifications of caladium.
点击查看大图
下载:
