Tissue Culture and Micro-tuber Induction of Dioscorea opposita, Jiu Jin Huang
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摘要: 利用山药珠芽进行种茎繁殖,对山药品种具有更新复壮作用;为降低山药种植成本,促进山药品种更新复壮,探索适宜水山药品种九斤黄微型块茎诱导的培养条件。通过不同浓度NAA/6-BA激素组合对茎尖诱导的作用,以及不同浓度NAA/KT激素组合、不同活性炭浓度、不同蔗糖浓度处理,筛选适宜微型块茎诱导的配方条件。结果表明:MS培养基中添加0.1 mgL-1NAA+2.0 mgL-16-BA为水山药九斤黄茎尖诱导最佳浓度,植株再生率达到了91.7%;九斤黄微型块茎诱导最适宜培养基配方为:MS基本培养基+0.1 mgL-1NAA + 1.0 mg L-1KT+2.0 gL-1AC+50.0 gL-1蔗糖+8.0 gL-1琼脂粉。再生微型块茎的种植仍然保持原品种种性,但外观商品性获得提高。Abstract: Using yam bulbils for tuber propagation not only benefits the rejuvenation of Dioscorea opposita but also reduces the planting cost. Thus, conditions of the micro-tuber induction for the yams were studied. The shoot tips were cultured in MS medium with additions of varying concentrations of NAA/6-BA. Different treatments using NAA/KT, active carbon and sucrose were applied for the experimentation. The results indicated the optimal medium composition for the shoot tip induction to be: MS + 0.1 mgL-1 NAA+ 2.0 mgL-1 6-BA+ 0.2 gL-1 AC+ 30.0 gL-1 sucrose+ 8.0 gL-1 agar.The resultant plant regeneration rate was 91.7%. The optimal medium formula for the micro-tuber induction was: MS +0.1 mgL-1 NAA+ 1.0 mgL-1 KT+ 2.0 gL-1 AC+ 50.0 gL-1 sucrose+ 8.0 gL-1agar.The regenerated plants from the micro-tubers not only maintained the characteristics of the parent species but also improved its commercial appealing.
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Key words:
- Dioscorea opposita /
- micro-tuber induction /
- tissue culture
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