黑麦草根系抗氧化系统和细胞质膜透性对土壤中芘胁迫的响应

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基金项目:

福建省自然科学基金项目(2010J01023)

Antioxidative Activities and CMP of Rye Grass Rhizotaxy under Pyrene Stress

1.
Institute of Soil and Fertilizer, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
2.
Research Centre of Agricultural Resource and Environment, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

摘要: 采用盆栽试验研究土壤中添加不同浓度芘对黑麦草根系抗氧化系统和细胞质膜透性的影响。结果表明:芘对黑麦草根系抗氧化系统和细胞质膜透性的影响主要表现在试验20 d时,100~400 mgkg-1芘浓度下黑麦草根系的CMP、MDA和SS含量显著增加,膜脂过氧化严重;同时CAT活性和非酶抗氧化剂GSH含量相应显著上升,以保护根细胞免受伤害。另外2种抗氧化酶POD活性和SOD活性则明显下降,其中POD活性下降显著。
- 黑麦草 /
- 根系 /
- 抗氧化酶 /
- 细胞质膜透性 /
- 芘胁迫
Abstract: Effects of varying Pyrene levels in soil on the antioxidative activities and cytoplasm membrane permeability (CMP) in the rhizotaxy of rye grass were studied. The results showed that the effect of the Pyrene stress appeared mostly 20d after treatment. The contents of CMP, MDA and SS in the roots increased significantly under 100-400 mgkg-1 Pyrene addition in the soil. The membrane lipids of the rye grass roots showed serious peroxidation. On the other hand, the catalase(CAT) activity and non-enzymatic antioxidant GSH content added dramatically to protect the root cells from injuries. The activities of peroxidase (POD) and superoxide dismutase(SOD) reduced under the Pyrene stress, with the POD activity decreased significantly.
- Rye grass /
- rhizotaxy /
- antioxidative enzyme /
- cytoplasm membrane permeability /
- Pyrene stress