摘要:
采用昆虫触角电位测定方法,以十字花科9种植物挥发性物质为对象,研究小菜蛾雌、雄成虫对不同浓度植物挥发性物质的触角电位反应。试验结果表明:六碳醇或六碳醛均能引起较强的EAG反应,如正己醛、反-2-己烯醛、正己醇,而萜烯类化合物如(1R)-(+)-α-蒎烯、α-萜品烯,(+)-3-莰烯、桉树脑和D-柠檬烯激发的EAG相对值较小。小菜蛾对正己醛、反-2-己烯醛、正己醇、庚醛、D-柠檬烯、α-萜品烯的EAG相对值随着浓度的升高逐渐增大,对(1R)-(+)-α-蒎烯、3-莰烯、桉树脑的EAG反应相对值随浓度的升高先减小后再增大。在所有植物挥发性物质中,雌蛾对反-2-己烯醛的EAG反应值最大,为331%,雄蛾对正己醇的EAG反应值最大,为298%。在同一浓度下,雌、雄蛾的触角相对反应值总体上差异不显著,且均未超过2倍。
Abstract:
Electroantennogram(EAG)was employed to record the electrophysiolog ical responses of the diamondback moth(DBM)adult to crucifer of nine volatiles.The results showed higher EAG responses of DBM to six carbon alcohol and aldehyde were displayed,such as 1-hexanol,hexanal,trans-2-hexen-1-al.However,the weaker EAG responses of DBM to terpenes such as(1R)-(+)-α-pinene,α-terpinene,(+)-3-carene,1,8-cineole and d-limonene were displayed.The EAG responses were enhanced with the increase in concentration,of 1-hexanol,hexanal,trans-2-hexen-1-al,heptanal,α-terpinene and d-limonene respectively.The EAG response to(1R)-(+)-α-pinene,(+)-3-carene and 1,8-cineole were decreased at first and then increased to the maximum with increasing concentrations.The highest EAG response in female was to trans-2-hexen-1-al and the EAG relative value was approximately 331%,and that in male was to 1-hexanol and the EAG relative value was approximately 298%.The maximal differences of EAG relative values of DBM responding to the same concentration of volatiles were no more than two fold.
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