荔枝体胚发生早期蛋白质组分变化的初步分析

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基金项目:

福建省亚热带果树及特种经济作物种质资源共享平台(2008N2001);国家教育部霍英东教育基金会高等学校青年教师基金项目(71026);国家自然科学基金项目(39870544)

Changes of Embryo Protein Components During Early Somatic Embryogenesis of Litchi(Litchi chinensis Sonn.)

1.
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
2.
Institute of Agricultural Engineering and Technology, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;
3.
Agricultural Bio-resource Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

摘要: 以‘下番枝'品种荔枝体胚发生早期7个阶段的胚性培养物为材料,采用SDS-PAGE蛋白质电泳方法分离蛋白质组分。试验结果表明:荔枝体胚发生早期存在着丰富的蛋白质组分,且各阶段之间的蛋白质组分存在着明显的差异;出现的6.13、5.08、4.02 kD小分子量的特异蛋白质,可能在荔枝体胚发生早期过程中起着重要的作用。
- 荔枝 /
- 体胚 /
- 体胚发生 /
- 蛋白质
Abstract: The protein components of seven embryogenic cultures were separated by SDS-PAGE during the early litchi somatic embryogenesis. The results showed that the protein components varied obviously during various stages of early somatic embryogenesis of litchi. Three low molecular weight proteins(6.13 kD、5.08 kD and 4.02 kD) emerged at the early developmental stages of somatic embryogenesis, which maybe played an important role in the process of the early somatic embryogenesis.
- Litchi chinensis Sonn /
- somatic embryo /
- somatic embryogenesis /
- protein

[1]	ZIMMERMAN J L. Somatic embryogenesis:a model for early development in higher plants[J]. Plant Cell, 1993, 5:1411-1423.
[2]	STASOLLA C, BOZHKOV P V, CHU T M,et al. Variation in transcript abundance during somatic embryogenesis in gymnosperms[J]. Tree Physiology, 2004, 24(10):1073-1085.

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