Optimization of Culture and Rapid Propagation for Phyllagathis fordii
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摘要: 应用正交试验方法研究6-BA,NAA及KT等3个因子对叶底红组培苗增殖系数的影响,同时考查了IBA、NAA和活性炭等3个因子对叶底红组培苗生根培养的影响,试验均按正交表L9(34)形成三因素、三水平的不同配比。结果表明:适宜叶底红增殖的培养基为MS＋6-BA 1.2 mg·L-1＋NAA 0.4 mg·L-1＋KT 0.1 mg·L-1,平均增殖系数3.62;适于生根的培养基为1/2MS+IBA 0.5mg·L-1+活性炭1.0 g·L-1。以泥炭土和珍珠岩(2∶1)作为移栽基质,移栽成活率可达90%以上。Abstract: Effects of hormones and activated carbon in medium on the proliferation and rooting of Phyllagathis fordii were studied for the culture optimization.Plant tissues from aseptic seedlings were used as the explants for the orthogonal design experiment [L9(34)].The result showed that (a) MS supplemented with 6-BA 1.2 mg·L-1＋NAA 0.4 mg·L-1＋KT 0.1 mg·L-1 was appropriate for the proliferation; (b) 1/2 MS supplemented with IBA 0.5 mg·L-1+activated carbon 1.0 g·L-1 was suitable for rooting; and (c) the survival rate were greater than 90% when the plantlets were transplanted to a soil medium containing 2/3 peat and 1/3 perlite.
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Key words:
- Phyllagathis fordii /
- tissue culture /
- rapid propagation /
- orthogonal experiment
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