摘要:
为对福建省马铃薯新品种(系)鉴定提供分子水平上的依据,利用筛选出的11对多态性较好的SSR标记对9份马铃薯新品种(系)及其主要亲本进行遗传分析并构建指纹图谱。结果表明,供试材料共扩增出89个等位位点,其中84个为多态性位点,多态性比率达94.38%。不同引物扩增出等位位点数5(STM1049)-15(STI027)个,平均8.09个。利用STI027、STI052、STI033、STI047、STM3023和STM0030共6对引物构建了供试品种(系)的SSR指纹图谱。其中引物STI027可以将13份品种(系)完全区分开,引物STM0030可以将10个品种(系)区分开。聚类分析表明,在相似系数0.628处,可将13份品种(系)分为四类,聚类结果与供试品种(系)的系谱来源有较好一致性。
Abstract:
For the purpose of new varieties(lines) identification at the molecular level, 11 pairs of SSR(Simple Sequence Repeats) primer markers were employed to analysis of genetic and construction of fingerprinting for 9 new potato varieties and its parents in Fujian.. The results showed that, 89 alleles loci were amplified, including 84 polymorphic alleles, and the ratio of polymorphism reached 94.38%. The numbers of alleles site amplified with different primers was 5-15, and the average value was 8.09. The fingerprinting of 13 potato varieties was constructed by 6 pairs of primers,STI027, STI052, STI033, STI047, STM3023, and STM0030. All the potato varieties were univocally identified using SSR primer STI027, and ten potato varieties were univocally identified using SSR primer STM0030. UPGMA cluster analysis of genetic similarity showed that all the materials were clustered into four groups at the genetic similarity of 0.628. The cluster analysis result was accordance with the genetic relationships.
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