香蕉枯萎病菌线粒体小亚基(mtSSU)rDNA的克隆及序列分析

郑荔; 兰成忠; 姚锦爱; 阮宏椿; 陈福如

香蕉枯萎病菌线粒体小亚基(mtSSU)rDNA的克隆及序列分析

1.
福州出入境检验检疫局, 福建福州 350002;
2.
福建省农业科学院植物保护研究所, 福建福州 350013

基金项目:

福州市科技计划项目(2010-N-67);福建省自然科学基金项目(2011J01114);福建省发改委高新技术项目(2011-352)

详细信息

通讯作者:
阮宏椿(1980-),女,硕士,助理研究员,研究方向:植物病害防治(Email:hcruan@126.com)

中图分类号: S435.32
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出版历程
- 收稿日期: 2012-06-07
- 修回日期: 2012-07-11
- 刊出日期: 2012-08-30

Cloning and Sequencing Analysis of Mitochondrial Small Subunit(mtSSU) rDNA Sequence of Fusarium oxysporum f.sp.cubense

1.
Fuzhou Entry-exit Inspection & Quarantine Bureau of the People's Republic of China, Fuzhou, Fujian 350002, China;
2.
Institute of Plant Protection Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

摘要

摘要: 为探讨镰刀菌属的系统发育情况,采用真核生物线粒体中核糖体小亚基基因(mtSSU rDNA)的通用引物,PCR扩增了2株不同生理小种(小种4和小种1)的香蕉枯萎病菌mtSSU rDNA序列,并对PCR产物进行了序列分析,同时利用MEGA4软件中的Neighbor-joining(N-J)法,对2个样品序列以及GenBank中登陆的镰刀菌属不同种及尖镰孢种不同专化型的mtSSU rDNA序列,构建聚类分析树状图。结果显示,所测的2株香蕉枯萎病菌mtSSU rDNA全长685 bp,两者之间同源性为99.70%。聚类分析表明,所测的2个样品和Fusarium oxysporum f.sp.cubense专化型在一个聚类组中,镰刀菌不同种及同种不同专化型间的mtSSU rDNA序列变异较大,说明该菌不同种及同种不同专化型间的遗传多样性丰富。
- 香蕉枯萎病菌 /
- 线粒体小亚基基因 /
- 基因克隆 /
- 序列分析 /
- 同源性
Abstract: To explore the phylogenetic correlations among the strains of Fusarium spp., the mitochondrial small subunit (mtSSU) rDNA genes from Fusarium oxysporum f.sp.cubense in two physiological races (race4 and race1) were amplified by polymerase chain reaction using the mtSSU rDNA gene universal primers. Furthermore, phylogenetic tree was constructed on the basis of the two race mtSSU rDNA sequences and other mtSSU rDNA sequences of Fusarium spp. from GenBank by neighbor-joining method. The results showed that lengths of the gene in two races were 685bp and sequence homology between the two races was 99.70%. Phylogenetic analysis suggested that the two sequences were clustered in the same group with the sequence of F. oxysporum f.sp.cubense in the database. Meanwhile, there were variations in the mtSSU rDNA sequences of inter-species and the same species with different specialized obviously, suggesting that the abundant genetic diversity of mtSSU rDNA gene sequences could be used in phylogenetic and evolutionary analyses of Fusarium spp.
- Fusarium oxysporum f.sp.cubense /
- Mitochondrial Small Subunit (mtSSU) rDNA /
- Gene cloning /
- Sequence analysis /
- Homology

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参考文献(3)

[1]	刘开辉,邓百万,陈文强,等.比较DNA序列分析不同猪苓菌种的亲缘关系[J].食用菌学报,2009,16(3):11-14.
[2]	刘连盟,王玲,黄雯雯,等.水稻稻曲病菌G蛋白β亚基基因的克隆、表达与序列分析[J].中国水稻科学,2010,24(4):353-359.
[3]	KERRY O DONNELL,H CORBY KISTLER,ELIZABETH CIGELNIL,et al.Multiple evolutionary origins of the fungus causing Panama disease of banana: Concordant evidence from unclear and mitochondrial gene genealogies[J]. Proceedings of the National Academy of Sciences of the United States of America,1998,95:2044-2049.