摘要:
根据GenBank登录的SCYLV CP基因序列,设计特异性引物,应用RT-PCR方法从黑皮果蔗病株中克隆甘蔗黄叶病毒外壳蛋白基因,扩增出608个核苷酸(nt)的特异片段。核苷酸和氨基酸序列分析结果表明,该片段包含甘蔗黄叶病毒(SCYLV)外壳蛋白基因的全长,编码196个氨基酸,与国内外已报道的SCYLV分离物同源性达96%以上;从构建的来自不同地区18个分离物的系统进化树得出本研究分离到的SCYLV-FJBadila属于BRA-PER基因型;对福建省果蔗种植区长泰、龙文、同安等地的果蔗进行采样检测,结果表明,福建省果蔗普遍受到SCYLV的侵染。
Abstract:
According to the SCYLV coat protein (CP) gene sequences from GenBank, the specific primers were designed to amplify SCYLV CP gene from Fujian chewingcane (Badila) by RT-PCR..The PCR product contained 608 nucleotides. The results of nucleotide and amino acid sequence analysis showed that the PCR product contained the full length of SCYLV coat protein, which coded 196 amino acids. The CP gene sequences in this test have more than 96% homologous with CP genes of other SCYLV isolates from China and other countries. Phylogenetic tree suggested that the eighteen isolates were classified into three types according to the nucleotides sequence of SCYLV CP. The SCYLV-FJBadila belong to the BRA-PER genotypes.We sampled and amplified the CP genes from the chewingcane fields in LongWen, ChangTai, TongAn, etc of Fujian province, the result showed that the chewingcanes in Fujian province were universal infected with SCYLV.
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