粳稻云引中一个受稻瘟病菌诱导的抗逆候选基因的克隆及表达分析

何炜; 连玲; 熊海清; 蔡秋华; 朱永生; 谢鸿光; 王颖姮; 张建福; 谢华安

粳稻云引中一个受稻瘟病菌诱导的抗逆候选基因的克隆及表达分析

何炜^1,2,3,4,
连玲^1,2,3,4,
熊海清^1,2,3,4,
蔡秋华^1,2,3,4,
朱永生^1,2,3,4,
谢鸿光^1,2,3,4,
王颖姮^1,2,3,4,
张建福^1,2,3,4, ,,
谢华安^1,2,3,4, ,

1.
福建省农业科学院水稻研究所, 福建福州 350019;
2.
农业部华南杂交水稻种质创新与分子育种重点实验室/ 福州国家水稻改良分中心/福建省作物分子育种工程实验室/福建省水稻分子育种重点实验室, 福建福州 350003;
3.
福建省作物种质创新与分子育种省部共建国家重点实验室培育基地, 福建福州 350003;
4.
水稻国家工程实验室, 福建福州 350003

基金项目:

国家"973"计划项目(2012CB723003);国家"863"计划项目(2011AA10A101);福建省杰出青年基金项目(2009J06011);福建省财政专项——福建省农业科学院科技创新团队建设项目(STIF-Y04)

详细信息

通讯作者:
张建福(1971-),男,博士,副研究员,主要从事水稻遗传学与分子育种研究(E-mail:jianfzhang@163.com)谢华安(1941-),男,研究员,中国科学院院士,主要从事水稻遗传育种研究(E-mail:huaanxie@yahoo.com.cn)

张建福(1971-),男,博士,副研究员,主要从事水稻遗传学与分子育种研究(E-mail:jianfzhang@163.com)谢华安(1941-),男,研究员,中国科学院院士,主要从事水稻遗传育种研究(E-mail:huaanxie@yahoo.com.cn)

中图分类号: S511
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出版历程
- 收稿日期: 2012-07-09
- 修回日期: 2012-08-03
- 刊出日期: 2012-09-30

Functional Analysis of a Candidate Gene in Relation to Stress Resistance Induced with Magnaporthe grisea from Japonica Variety Yunyin

HE Wei^1,2,3,4,
LIAN Ling^1,2,3,4,
XIONG Hai-qing^1,2,3,4,
CAI Qiu-hua^1,2,3,4,
ZHU Yong-sheng^1,2,3,4,
XIE Hong-guang^1,2,3,4,
WANG Ying-heng^1,2,3,4,
ZHANG Jian-fu^{1,2,3,4
, ,},
XIE Hua-an^{1,2,3,4
, ,}

1.
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350019, China;
2.
Key Laboratory of Germplasm Innovation and Molecular Breeding for Hybrid Rice of South China, Ministry of Agriculture, P.R China/Fuzhou branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/ Fujian Key Laboratory of Rice Molecular Breeding, Fuzhou, Fujian 350003, China;
3.
Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Sciences & Technology, P.R.China, Fuzhou, Fujian 350003, China;
4.
National Rice Engineering Laboratory of China, Fuzhou, Fujian 350003, China

摘要

摘要: 在对粳稻云引受稻瘟病菌诱导的基因芯片分析的基础上,克隆了其中一个受稻瘟病菌诱导后表达量显著变化的基因。序列比对结果显示,该基因与籼稻AF529237.2序列及普通小麦 X51608.1 序列分别具有99.0% 与88.0% 的同源性,与磷酸核酮糖激酶的活性相关,在糖代谢中起到重要的作用。构建该基因的系统进化树,结果表明在小麦、大麦、玉米等禾本科作物中也有其同源序列,并且同源性较高。推测该基因可能参与磷酸核酮糖和磷酸戊糖的代谢,执行磷酸核酮糖激酶的功能,并构建了该基因的过表达载体、干扰表达载体与瞬时表达载体。
- 粳稻云引 /
- 稻瘟病菌 /
- 抗逆候选基因 /
- 功能分析
Abstract: Based on gene chip of japonica rice cv. Yunyin induced with Magnaporthe grisea, one of the candidate genes up-regulated remarkably by Sichuan43's isolation was cloned. Sequence and phylogenetic tree analyses suggest that the gene is closest relative to Oryza sativa L. AF529237.2 and Tritieum aestivum X51608.1 with 99.0% and 88.0% identity, and may relate to phosphoribulo kinase which is a critical enzyme in calvin cycle. Subsequently, the overexpression, RNAi expression and transient expression vector were constructed to obtain corresponsive transgenic lines, respectively. All these transgenic lines generated were used to assess the function of the gene in the stress response.
- Japonica rice Yunyin /
- Magnaporthe grisea /
- candidate gene /
- functional analysis

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参考文献(3)

[1]	ZEIGLER R S,LEONG S A,TENG P S.Rice Blast Disease[M].CAB International and IRRI,Wallingford,United Kingdom,1994:16-26.
[2]	SILUD D,NOTTENGHEM J L,THARREAU D. Evidence of a gene-for-gene relationship in the Oryza sativa-Magnaporthe grisea pathosystem [J]. Phytopathology, 1992,82:557-580.
[3]	RUMPHO M E,POCHAREDDY S,WORFUL J M,et al. Molecular Characterization of the Calvin Cycle Enzyme Phosphoribulokinase in the Stramenopile Alga Vaucheria litorea and the Plastid Hosting Mollusc Elysia chlorotica[J]. Molecular Plant,2009,2(6):1384-1396.