猪嵴病毒福建株3D基因的克隆及遗传进化分析

陈如敬; 吴学敏; 车勇良; 王隆柏; 魏宏; 庄向生; 刘玉涛; 严山; 周伦江

猪嵴病毒福建株3D基因的克隆及遗传进化分析

1.
福建省农业科学院畜牧兽医研究所/福建省畜禽疫病防治工程技术研究中心, 福建福州 350013

基金项目:

国家公益性行业(农业)科研专项(NYHYZX07-034);福建省财政专项——福建省农业科学院科技创新团队建设项目(STIF-Y02)

详细信息

通讯作者:
周伦江(1973-),男,博士,副研究员,研究方向:畜禽传染病(E-mail:lunjiang@163.com)

中图分类号: S852
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出版历程
- 收稿日期: 2012-07-10
- 修回日期: 2012-08-02
- 刊出日期: 2012-09-30

Cloning and Evolution Analysis of the 3D Gene of Porcine Kobuvirus in Fujian

1.
Institute of Animal Husbandry and Veterinary Medicine/Fujian Animal Diseases Control Technology Development Center, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

摘要

摘要: 根据GenBank中登录的猪嵴病毒(porcine kobuvirus, PKV)3D基因序列特征设计特异性引物,采用RT-PCR方法从福建省某猪场采集腹泻粪便样品和小肠组织混合物中扩增猪嵴病毒3D基因,将扩增后的目的片段克隆后进行序列测定。结果表明,所扩增的目的片段编码有完整的3D开放阅读框,全长为1 407 bp,编码有468个氨基酸。运用生物信息学软件,将获得3D基因序列和GenBank的猪嵴病毒株3D基因序列进行分析比较,该毒株的3D基因序列和SH-W-CHN/2010/China毒株的核苷酸同源性最高,为93.6%,与WH1株核苷酸同源性最低,为92%。从遗传进化上看,猪嵴病毒和其他中国株在遗传进化上处在一个同一分支,匈牙利分离株处在另一分支,表明猪嵴病毒可能在欧亚大陆上各自独立进化。
- 猪嵴病毒 /
- 3D基因 /
- 遗传进化分析
Abstract: The 3D gene of porcine kobuvirus was amplified by RT-PCR from tissues of piglets association with diarrhea from Fujian and then sequenced. The results showed that the porcine kobuvirus 3D gene was 1 407 bp in length, coding an open reading frame (ORF) containing 468 amino acids.Compared with the published PKV 3D genes by the bioinformatics software, the sequenced gene shared the highest homogeneity with SH-W-CHN/2010/China strain of 93.6%. In the phylogenetic tree, the porcine kobuvirus Fujian strain was at the same branch with other strains isolated in China while the Hungarian isolates share another branch, which indicated that the porcine kobuvirus had its own evolution status in different continents.
- porcine kobuvirus /
- 3D gene /
- evolution analysis

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参考文献(3)

[1]	REUTER G, BOROS A, PANKOVICS P. Kobuviruses-a comprehensive review [J]. Rev Med Virol, 2011, 21(1):32-41.
[2]	REUTER G, BOLDIZS R A, PANKOVICS P. Complete nucleotide and amino acid sequences and genetic organization of porcine kobuvirus, a member of a new species in the genus Kobuvirus, family Picornaviridae [J]. Arch Virol, 2009, 154(1):101-108.
[3]	陈如敬,吴学敏,车永良,等.猪流行性腹泻病毒FJ-11A株的分离与ORF3基因序列分析[J].福建农业学报,2011,26(6):947-951.