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水产养殖废水脱氮除磷微藻的筛选

贾纬 聂毅磊 陈宏 罗立津 乐占线 庄鸿 郑军荣

贾纬,聂毅磊,陈宏,等. 水产养殖废水脱氮除磷微藻的筛选 [J]. 福建农业学报,2021,36(2):243−248 doi: 10.19303/j.issn.1008-0384.2021.02.016
引用本文: 贾纬,聂毅磊,陈宏,等. 水产养殖废水脱氮除磷微藻的筛选 [J]. 福建农业学报,2021,36(2):243−248 doi: 10.19303/j.issn.1008-0384.2021.02.016
JIA W, NIE Y L, CHEN H, et al. Microalgae for Effective Nitrogen and Phosphorus Removal from Aquaculture Effluence [J]. Fujian Journal of Agricultural Sciences,2021,36(2):243−248 doi: 10.19303/j.issn.1008-0384.2021.02.016
Citation: JIA W, NIE Y L, CHEN H, et al. Microalgae for Effective Nitrogen and Phosphorus Removal from Aquaculture Effluence [J]. Fujian Journal of Agricultural Sciences,2021,36(2):243−248 doi: 10.19303/j.issn.1008-0384.2021.02.016

水产养殖废水脱氮除磷微藻的筛选

doi: 10.19303/j.issn.1008-0384.2021.02.016
基金项目: 福建省科技计划星火项目(2017S0006)
详细信息
    作者简介:

    贾纬(1976−),男,助理研究员;主要从事环境微生物研究(E-mail:3773475@qq.com

    通讯作者:

    聂毅磊(1976−),男,副研究员;主要从事微生物技术研究(E-mail:244701760@qq.com

  • 中图分类号: X 713

Microalgae for Effective Nitrogen and Phosphorus Removal from Aquaculture Effluence

  • 摘要:   目的  筛选对养殖废水具有脱氮除磷效果的微藻藻种。  方法  选取小球藻JY-1(Chlorella sp.JY-1)、小球藻SY-4(Chlorella sp.SY-4)以及链带藻SH-1(Desmodesmus sp. SH-1)等3株微藻为研究对象,研究其对水产养殖废水脱氮除磷的效果。  结果  培养5 d,JY-1、SY-4和SH-1在7‰±1‰盐度养殖废水中的细胞含量分别为1.56×107、1.47×107、6.62×106个·mL−1;JY-1、SY-4和SH-1对养殖废水中总氮的去除率分别为50.36%、41.51%和49.74%;氨态氮(NH4+-N)的去除率分别为96.29%、84.92%和96.65%;硝态氮(NO3-N)的去除率分别为15.84%、3.69%和12.56%;总磷(PO43-P)的去除率分别为93.51%、82.38%和94.25%;对亚硝态氮的去除效果不明显;3株藻在5‰、10‰、20‰和30‰盐度的培养基中均可以正常生长。JY-1在养殖废水中的生长能力和对水体净化能力均优于小球藻SY-4和链带藻SH-1。  结论  小球藻JY-1对水产养殖废水具有较好的脱氮除磷效果。本研究为应用微藻进行养殖废水脱氮除磷处理提供了参考数据。
  • 图  1  3种微藻在不同盐度培养基中生长的最终细胞含量

    注:不同藻种之间无相同小写字母者表示差异达到显著水平;无相同大写字母者表示差异达到极显著水平。表1~5同。

    Figure  1.  Final cell densities of microalgae in media of varied salinities

    Note: Data with different lowercase letters indicate significant difference; data with different uppercase letters indicate very significant difference.The same as table 1-5.

    图  2  3株微藻在水产养殖废水中的细胞含量曲线图

    Figure  2.  Growth of microalgae in aquaculture wastewater

    表  1  3株微藻对水产养殖废水中总氮的去除效果

    Table  1.   Efficiency of total nitrogen removal by various microalgae (单位: mg·L−1

    藻种
    Species
    0 d 1 d 2 d 3 d 4 d 5 d5 d去除率
    removal rate/%
    SH-1 10.757±0.06 a 8.290±0.06 Dd 7.230±0.07 Cc 5.693±0.06 Cc 5.530±0.03 Cc 5.407±0.06 Cc 49.74
    JY-1 10.730±0.06 a 8.690±0.03 Cc 7.323±0.03 Cc 5.707±0.08 Cc 5.593±0.08 Cc 5.327±0.07 Cc 50.36
    SY-4 10.760±0.07 a 9.853±0.06 Bb 8.877±0.09 Bb 7.160±0.09 Bb 6.530±0.07 Bb 6.293±0.06 Bb 41.51
    CK 10.743±0.06 a 10.550±0.07 Aa 10.430±0.08 Aa 10.363±0.08 Aa 10.307±0.05 Aa 10.210±0.05 Aa 4.96
    下载: 导出CSV

    表  2  3株微藻对水产养殖废水中氨氮的去除效果

    Table  2.   Efficiency of NH4+-N removal by various microalgae (单位: mg·L−1

    藻种
    Species
    0 d 1 d 2 d 3 d 4 d 5 d5 d去除率
    removal rate/%
    SH-1 2.790±0.02 a 1.037±0.03 Dd 0.357±0.03 Cc 0.127±0.00 Cc 0.103±0.00 Cc 0.093±0.00 Cc 96.65
    JY-1 2.787±0.02 ab 1.347±0.05 Cc 0.310±0.03 Cc 0.127±0.01 Cc 0.117±0.00 Cc 0.103±0.00 Cc 96.29
    SY-4 2.763±0.02 ab 1.967±0.03 Bb 1.180±0.03 Bb 0.777±0.03 Bb 0.543±0.03 Bb 0.417±0.02 Bb 84.92
    CK 2.733±0.02 b 2.677±0.02 Aa 2.603±0.03 Aa 2.477±0.02 Aa 2.397±0.02 Aa 2.347±0.02 Aa 14.15
    下载: 导出CSV

    表  3  3株微藻对水产养殖废水中硝态氮的去除效果

    Table  3.   Efficiency of NO3-N removal by various microalgae (单位: mg·L−1

    藻种
    species
    0 d 1 d 2 d 3 d 4 d 5 d5 d去除率
    removal rate/%
    SH-1 3.980±0.03 a 3.937±0.02 a 3.870±0.03 b 3.723±0.02 Bc 3.623±0.02 Cc 3.480±0.03 Cc 12.56
    JY-1 3.977±0.02 a 3.920±0.02 a 3.850±0.03 b 3.587±0.03 Cd 3.457±0.02 Dd 3.347±0.02 Dd 15.84
    SY-4 3.937±0.02 a 3.950±0.02 a 3.910±0.01 ab 3.877±0.02 Ab 3.850±0.01 Bb 3.827±0.01 Bb 3.69
    CK 3.957±0.01 a 3.957±0.02 a 3.940±0.01 a 3.943±0.02 Aa 3.937±0.02 Aa 3.933±0.01 Aa 0.59
    下载: 导出CSV

    表  4  3株微藻对水产养殖废水中亚硝态氮的去除效果

    Table  4.   Efficiency of NO2-N removal by various microalgae (单位: mg·L−1

    藻种
    species
    0 d 1 d 2 d 3 d 4 d 5 d5 d去除率
    removal rate/%
    SH-1 0.100±0.00 a 0.103±0.00 a 0.110±0.00 a 0.130±0.01 Ab 0.143±0.00 Bb 0.163±0.00 Ab −63.33
    JY-1 0.100±0.00 a 0.107±0.00 a 0.107±0.00 ab 0.143±0.00 Aa 0.157±0.00 Aa 0.177±0.00 Aa −76.67
    SY-4 0.097±0.00 a 0.100±0.00 a 0.100±0.00 b 0.107±0.00 Bc 0.107±0.00 Cc 0.117±0.00 Bc −20.69
    CK 0.097±0.00 a 0.100±0.00 a 0.103±0.00 ab 0.100±0.00 Bc 0.103±0.00 Cc 0.103±0.00 Bd −6.90
    下载: 导出CSV

    表  5  3株微藻对水产养殖废水中总磷的去除效果

    Table  5.   Efficiency of total phosphate removal by various microalgae (单位: mg·L−1

    藻种
    species
    0 d 1 d 2 d 3 d 4 d 5 d5 d去除率
    removal
    rate/%
    SH-1 0.870±0.01 a 0.430±0.02 Cc 0.143±0.01 Dd 0.053±0.00 Cc 0.050±0.01 Cc 0.050±0.00 Cc 94.25
    JY-1 0.873±0.02 a 0.427±0.02 Cc 0.187±0.01 Cc 0.057±0.00 Cc 0.053±0.00 Cc 0.057±0.00 Cc 93.51
    SY-4 0.870±0.02 A 0.627±0.02 Bb 0.433±0.01 Bb 0.243±0.02 Bb 0.197±0.01 Bb 0.153±0.00 Bb 82.38
    CK 0.847±0.00 a 0.847±0.00 Aa 0.843±0.00 Aa 0.847±0.01 Aa 0.843±0.01 Aa 0.843±0.00 Aa 0.39
    下载: 导出CSV
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  • 收稿日期:  2019-08-29
  • 修回日期:  2020-10-27
  • 网络出版日期:  2020-11-24
  • 刊出日期:  2021-02-28

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