闽江流域福州过境段大肠杆菌毒素基因的定性定量分析与季节性变化

基金项目:

福建省环保科技项目(2006)

Multiplex-PCR analyses and seasonal changes of virulence genes of E.coli isolated from Min river watersheds in Fuzhou

1.
College of Life Science, Fujian Normal University, Fuzhou, Fujian 350108, China;
2.
Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;
3.
Fuzhou Environmental Monitoring Central Station, Fuzhou, Fujian 350003, China;
4.
Fujian Environment Protection Bureau, Fuzhou, Fujian 350003, China;
5.
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

摘要: 分别于不同季节在闽江流域福州过境段的洪山桥和解放大桥断面采集水样,进行大肠杆菌的分离、鉴定,用多元PCR方法对不同季节分离的大肠杆菌毒素基因的时空分布进行定性与定量分析。从分离的2015株粪大肠杆菌中发现840株潜在的致病性大肠杆菌,分别属于肠出血性大肠杆菌(EHEC)、肠聚集性大肠杆菌(EAEC)、肠致病性大肠杆菌(EPEC)和肠产毒性大肠杆菌(ETEC);检出的10种毒素基因中,以扩散性黏附素基因(aidA-1)、热稳定性肠毒素基因(astA)和耐药性因子(sepA)为最多;细菌总量、大肠杆菌总数和致病性大肠杆菌总数在冬季较少,春季较多,夏秋季节达到全年数量的高峰;潜在的致病性大肠杆菌毒素基因的种类和数量也是在冬季最少,春夏季上升,秋季最多。
- 闽江流域 /
- 大肠杆菌 /
- 毒素基因 /
- 多元PCR
Abstract: Toxic genes of the potential pathogenic E.coli collected in different seasons from Min River watersheds in Fuzhou were analyzed using Multiplex-PCR.The results indicated that 840 out of 2015 E.coli isolates were potential pathogens classified as EHEC,EAEC,EPEC and ETEC groups.Ten virulence genes were detected with dominant genes,aidA-1,astA and sepA.The numbers of bacteria,E.coli and E.coli carrying virulence genes,increased in the spring and summer,peaked in the fall,and decreased in the winter.
- Min River /
- E.coli /
- virulence gene /
- multiplex-PCR

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