水禽源禽1型副黏病毒强毒RT-PCR方法的建立

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基金项目:

国家自然科学基金项目(30671564);国家“973”重大基础研究专项子课题(2005CB523001);福建省科技重大专项子课题(2006NZ0003-2)

Development of RT-PCR for detecting virulent strains of waterfowl-origin avian paramyxovirus type 1

1.
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
2.
Fujian Animal Diseases Control Technology Development Center, Fuzhou, Fujian 350013, China;
3.
Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China

摘要: 参照GenBank上登录的水禽源禽1型副黏病毒强毒株F基因序列设计引物,建立检测水禽源禽1型副黏病毒强毒株的RT-PCR方法。该方法能从水禽源禽1型副黏病毒强毒株扩增出1条400 bp的特异片段,从水禽源禽1型副黏病毒弱毒、鸭瘟病毒、鸭1型肝炎病毒、减蛋综合征病毒、传染性法氏囊病病毒、H9亚型禽流感病毒和禽多杀性巴氏杆菌等均不能扩增出目的片段。敏感性试验显示该RT-PCR方法最低可检测出10 pg的病毒核酸。因此,该RT-PCR方法可用于水禽源禽1型副黏病毒强毒的临床诊断。
- 水禽源禽1型副黏病毒 /
- 强毒株 /
- RT-PCR
Abstract: An RT-PCR method was developed to detect virulent strains of waterfowl-origin avian paramyxovirus type 1(vwAPMV-1).The primers were designed according to the sequences of the fusion protein gene of vwAPMV-1s available at the GenBank.The result showed that the 400 bp of specific fragments were amplified in vwAPMV-1.However,negative results were obtained from Pasteurella multocide,EDS,DEV,IBDV,H9N2 influenza virus,DHV-1 and low virulent strains of waterfowl-origin avian paramyxovirus type 1.And,the 10pg of vwAPMV-1 RNA was amplified by the sensitivity test for the RT-PCR.The results indicated that the RT-PCR could be used for vwAPMV-1 detection.
- waterfowl-origin avian paramyxovirus type 1 /
- virulent strains /
- RT-PCR