氟苯尼考人工抗原的合成与鉴定

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名

邮箱

手机号码

标题

留言内容

验证码

基金项目:

山东省自然科学基金项目(Y2007D32)

Synthesis and identification of florfenicol antigen

1.
College of Animal Science and Technology, Shandon Agricultural University, Tai'an, Shandong 271000, China;
2.
Liding Biotechnology Institute of Shandong Province, Ji'nan, Shandong 250001, China

摘要: 采用混合酸酐(MA)法,将氟苯尼考(FFC)分别与牛血清白蛋白(BSA)和卵清蛋白(OVA)连接,制备人工免疫抗原FFC-HS-BSA和包被原FFC-HS-OVA,经紫外扫描(UV)、凝胶电泳(SDS-PAGE)、红外扫描光谱(IR)和动物免疫试验证实人工抗原合成成功。
- 氟苯尼考 /
- 人工抗原 /
- 混合酸酐法 /
- 凝胶电泳
Abstract: The immunogen,FFC-HS-BSA(florfenicol-bovine serum albumin),and the coating antigen FFC-HS-OVA(florfenicol-ovalbumin)were synthesized by using the mixed anhydride method.The man-made antigens were identified and certified by UV absorption,SDS-PAGE electrophoresis and animal immunization test.The results showed the process was successful.All antigens made had the correct haptens and carrier proteins.
- Florfenicol /
- synthesized antigen /
- mixed anhydride method /
- gel electrophoresis

[1]	HORSBERG T E,MARTINSEN B,VARMA K J.The disposition of 14C-florfenicol in Atlantic salmon[J].Aquaculture,1994,122:97-106.
[2]	RUSSEL H A.Liquid Chromatographic Analysis of Chloramphenicol in Bovine Muscles[J].Chroma-tographia,1978,11:341-343.
[3]	GONZALEZ-CASADO A,NAVAS N,DEL OLMO M.Determination obiosphnol a inwater bymicro liquid-liquid extraction followeby silylation and gas chromatography-mass spectrometry anaysis[J].Chromatogr Sci,1998,36(11):565-567.
[4]	荣康泰,徐勤惠,李根令,等.半抗原与载体的最佳分子结合比[J].中国生物化学与分子生物学报,1985,1(2):27-31.
[5]	杨利国,胡少昶,为平华,等.酶免疫测定技术[M].南京:南京大学出版社,1998:279-284.

点击查看大图