农药厂不同生境中甲胺磷降解细菌的分离、筛选与鉴定

官雪芳; 刘波; 林斌; 林抗美; 马丽娜

农药厂不同生境中甲胺磷降解细菌的分离、筛选与鉴定

官雪芳^1,2,
刘波^1, ,,
林斌²,
林抗美¹,
马丽娜¹

1.
福建省农业科学院农业生物资源研究所, 福建, 福州, 350003;
2.
福建省农业科学院农业工程技术研究所, 福建, 福州, 350003

基金项目:

国家水体污染控制与治理科技重大专项(2008ZX07425-002);福建省科技计划项目(2005Y008)

详细信息

通讯作者:
刘波(1957-),男,博士,研究员,从事微生物生物技术和微生物农药研究(E-mail: fzliubo@163.com)

中图分类号: X172
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出版历程
- 收稿日期: 2008-10-13
- 修回日期: 2009-02-05
- 刊出日期: 2009-02-15

Isolation,screening and identification of methamidoghos-degrading bacteria from habitats at a pesticide factory

1.
Agricultural Bioresources Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;
2.
Institute of Agricultural Engineering Technology;Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China

摘要

摘要: 对福建省福农生化有限公司不同生境中的甲胺磷降解细菌进行分离,用磷脂脂肪酸分析方法对菌进行鉴定,同时对降解细菌的生境特征进行分析。结果表明:对甲胺磷有降解作用的细菌共有22个属、28个种、67株菌株;在沟中污泥中分离到的降解细菌有8个属、12个种、35株菌株,其种类和数量多于其他的生境;而在各类降解细菌中,芽孢杆菌属和假单胞菌属分别有21株和19株,占分离到细菌总量的59.7%,其中的蜡状芽孢杆菌(Bacillus cereus)和恶臭假单胞菌(Pseudomonas putida)在以上3种生镜中大量存在。
- 甲胺磷 /
- 农药降解细菌 /
- 不同生境 /
- 分离 /
- 筛选 /
- 鉴定
Abstract: To study methamidoghos-degrading bacteria in different habitats at a pesticide factory,soil samples from sewage,drainage ducts and surroundings at Fu-Nong Biochemistry Co.,Ltd in Fujian were collected for the isolation.Sixty-seven bacterial stains were isolated employing media containing high concentration of methamidoghos followed by identification using the fatty-acid analysis.They were determined to belong to 22 genera.In the 3 different types of habitats,35 microcial stains belonging to 8 genera were found.The numbers of Bacillus and Pseudomonas strains were 21 and 19,respectively.In combination,they amounted to 59.7% of the total.Among them,Bacillus cereus and Pseudomonas putida could grow in soils of all the sampling sites.
- methamidophos /
- degrading bacterium /
- different habitats /
- isolation /
- screening /
- identification

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参考文献(2)

[1]	STEGER K,JARVIS A,SMARS S,et al.Comparison of signature lipid methods to determine microbial community structure in compost[J].Journal of Microbiological Methods,2003,55(2):371-382.
[2]	PCRKOVICH B S,ANDERSON T A,Kruger E L,et al.Enhanced Mineralization of C14 Atrazine in Koehia Scoparia Rhizosphere Soil from a Pesticide Contaminated Soil[J].Pestic Sci,1996,46:391-396.