Variation in microspore-derived plant ploidy of Chinese cabbage with different maturation characteristics
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摘要: 对不同熟性的大白菜小孢子植株群体的倍性变异进行观察,研究利用DNA流式细胞仪测定、形态学特征鉴定大白菜小孢子植株群体的倍性变异.结果表明,小孢子植株是倍性水平不同的混合群体;不同熟性的大白菜小孢子植株群体的倍性分布不同,中晚熟大白菜小孢子植株双单倍体比例最高,春大白菜最低;单倍体植株在组培无性繁殖中部分会自然加倍;DNA流式细胞仪和形态学方法均可以鉴定群体的倍性水平.Abstract: Variation of microspore-derived plant ploidy of Chinese cabbage with different maturation characteristics was investigated by means of DNA flow cytometry and morphological identification.The results showed that regenerated populations from the microspores were generally mixed ploidy.The ploidy distribution varied with maturing accessions.The microspore plants of the middle-to-late-maturing Chinese cabbage had the highest proportion of double-haploid,and the spring Chinese cabbage the lowest among all tested.Some haploid plantlets doubled naturally in tissue culture.It appeared that DNA flow cytometry and morphological observation could be used to determine the ploidy level.
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Key words:
- Chinese cabbage /
- microspore-derived plants /
- ploidy variation /
- ploidy identification
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[1] 周伟军,毛碧增,唐桂香,等.甘蓝型油菜小孢子再生植株染色体倍数检测研究[J].中国农业科学,2002,35(6):724-727. [2] FARNHAM M W,CANIGLIA E J,THOMAS C E.Efficient ploidy determination of anther-derived broccoli[J].Hortscience,1998,32(2):323-327. [3] 胡含,郗子英,贾双娥.小麦花粉愈伤组织植株体细胞的变异[J].遗传学报,1978,5(1):23-30. -
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