甜菜单胚细胞质雄性不育系与保持系的育性鉴定及筛选

赵雅儒; 邳植; 阚文亮; 吴则东

doi:10.19303/j.issn.1008-0384.2023.04.004

甜菜单胚细胞质雄性不育系与保持系的育性鉴定及筛选

doi: 10.19303/j.issn.1008-0384.2023.04.004

赵雅儒^{1, 2,},
邳植^{1, 2},
阚文亮³,
吴则东^{1, 2, ,}

1.
黑龙江大学现代农业与生态环境学院，黑龙江哈尔滨　150080
2.
黑龙江省普通高校甜菜遗传育种重点实验室（黑龙江大学），黑龙江哈尔滨　150080
3.
黑龙江省农垦科学院经济作物研究所，黑龙江哈尔滨　150080

基金项目: 国家现代农业产业技术体系项目（CARS-170111）

详细信息

作者简介:
赵雅儒（1998−），女，硕士研究生，研究方向：甜菜遗传及分子育种的研究（E-mail：931178094@qq.com）

通讯作者:
吴则东（1972−），男，博士，研究员，研究方向：甜菜遗传及分子育种的研究（E-mail：1997009@hlju.edu.cn）

中图分类号: S566.3
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- 被引次数: 0
出版历程
- 收稿日期: 2022-02-24
- 修回日期: 2022-08-04
- 网络出版日期: 2023-04-14
- 刊出日期: 2023-04-28

Certifying Heredity Purity of Monogerm Cytoplasmic Male Sterile and Maintainer Sugar Beet

ZHAO Yaru^{1, 2
,},
PI Zhi^{1, 2},
KAN Wenliang³,
WU Zedong^{1, 2
, ,}

1.
College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin, Heilongjiang　150080, China
2.
Key Laboratory of Sugar Beet Genetic Breeding(Heilongjiang University), Harbin, Heilongjiang　150080, China
3.
Heilongjiang Academy of Land Reclamation Sciences, Harbin, Heilongjiang　150080, China

摘要

摘要: 目的提高甜菜单胚细胞质雄性不育系与保持系的育性纯度。方法对课题组现有的40对（共计620颗个体植株）甜菜单胚细胞质雄性不育系与保持系进行分子标记育性鉴定，记录鉴定结果中显示不育系中混有的保持系植株和保持系中混有的不育系植株的对应编号；在田间进行形态学验证，同时剔除混杂株。结果利用分子标记育性鉴定共检测到92颗混杂植株的DNA，其中73颗为保持系中混有不育植株，19颗为不育系中混有可育植株；在田间进行形态学育性鉴定时，观察花粉鉴定植株育性的同时观察植株的粒性，共有122株个体植株发生混杂（包含分子标记育性鉴定为混杂的92颗植株），即73株为保持系混杂，49株为不育系混杂。在田间验证时发现还有多胚植株混杂，可能是在种子清选过程中，有一定的概率混进了多胚种子。结论通过田间对混杂植株的剔除，即保证了不育系与保持系的育性及粒性纯度，又为这40对甜菜单胚细胞质雄性不育系与保持系的遗传多样性分析提供了前提条件，奠定了配制二元不育系的基础。
- 甜菜 /
- 分子标记 /
- 育性鉴定 /
- 形态学 /
- 筛选
Abstract: Objective To improve the fertility purity of beet monogerm cytoplasmic male sterile lines and maintainers. Method Forty pairs of molecular markers from a collection of 620 monogerm cytoplasmic male sterile and maintainer sugar beet germplasms were identified.All genetically mixed markers in the sterile and maintainer plants were given specific codes and recorded.Subsequently, a morphological observation was carried out in the field to exclude genetically impure plants. Result The verification of heredity purity by molecular markers revealed 92 DNA including 73 in the maintainers mixed with that of the sterile plant and 19 in the sterile lines contaminated with that belonged to the maintainer.The field examination on pollens and grains identified 122 plants (as compared to 92 isolated by the molecular marker verification) with 73 maintainers and 49 sterile lines to be promiscuous.In addition, polygerm plants were unexpectedly found in the lot that could have grown from the accidentally included seeds. Conclusion By excluding adulterated plants in the field, the purity on heredity of sterile and maintainer lines could be assured.The procedure would provide the prerequisite essential for accurate genetic diversity determination and successful preparation of binary sterile sugar beet lines.
- Sugar beet /
- molecular marker /
- heredity certification /
- morphology /
- screening

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图 1 田间种植示意图

Figure 1. Schematic diagram of sugar beet planting in the field

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图 2 甜菜单胚细胞质雄性不育系15与保持系16的分子标记育性鉴定条带

Figure 2. Molecular identifications for Male Sterile Line No.15 and Maintainer No.16

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图 3 甜菜单胚细胞质雄性不育系75与保持系76的分子标记育性鉴定条带

Figure 3. Molecular identifications for Male Sterile Line No.75 and Maintainer No.76

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表 1 40对供试材料序号及名称

Table 1. Codes and names of 40 pair test materials

序号 Serial number	名称 Name	序号 Serial number	名称 Name	序号 Serial number	名称 Name	序号 Serial number	名称 Name
1	HD-CMS1	21	HD-CMS11	41	HD-CMS21	61	HD-CMS31
2	HD-O1	22	HD-O11	42	HD-O21	62	HD-O31
3	HD-CMS2	23	HD-CMS12	43	HD-CMS22	63	HD-CMS32
4	HD-O2	24	HD-O12	44	HD-O22	64	HD-O32
5	HD-CMS3	25	HD-CMS13	45	HD-CMS23	65	HD-CMS33
6	HD-O3	26	HD-O13	46	HD-O23	66	HD-O33
7	HD-CMS4	27	HD-CMS14	47	HD-CMS24	67	HD-CMS34
8	HD-O4	28	HD-O14	48	HD-O24	68	HD-O34
9	HD-CMS5	29	HD-CMS15	49	HD-CMS25	69	HD-CMS35
10	HD-O5	30	HD-O15	50	HD-O25	70	HD-O35
11	HD-CMS6	31	HD-CMS16	51	HD-CMS26	71	HD-CMS36
12	HD-O6	32	HD-O16	52	HD-O26	72	HD-O36
13	HD-CMS7	33	HD-CMS17	53	HD-CMS27	73	HD-CMS37
14	HD-O7	34	HD-O17	54	HD-O27	74	HD-O37
15	HD-CMS8	35	HD-CMS18	55	HD-CMS28	75	HD-CMS38
16	HD-O8	36	HD-O18	56	HD-O28	76	HD-O38
17	HD-CMS9	37	HD-CMS19	57	HD-CMS29	77	HD-CMS39
18	HD-O9	38	HD-O19	58	HD-O29	78	HD-O39
19	HD-CMS10	39	HD-CMS20	59	HD-CMS30	79	HD-CMS40
20	HD-O10	40	HD-O20	60	HD-O30	80	HD-O40

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表 2 40对供试材料取样编号

Table 2. Specimen codes of 40 pair test materials

序号 Serial number	名称 Name	编号 Number
1	HD-CMS1	1-1	1-2	1-3	1-4	1-5	1-6	1-7	1-8	1-9
2	HD-O1	2-1	2-2	2-3	2-4	2-5	2-6	2-7	2-8	2-9

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表 3 分子标记育性鉴定与形态学育性鉴定混杂株数目比较

Table 3. Number of plants with mixed genes determined by molecular and morphological verifications （单位：颗）

混杂类型 Mixed type	分子标记育性鉴定 Identification of fertility by molecular markers	田间形态学育性鉴定 Morphological fertility identification in the field
		与分子标记鉴定结果相同 With the same results	与分子标记鉴定结果不同 With the different rseults
		与分子标记鉴定结果相同 With the same results	多胚植株 Polyembryony plant	细胞核育性混杂 The nuclei are promiscuous
不育系混杂 Mixed sterile lines	19	19	12	18
保持系混杂 Mixed Maintainer line	73	69	0	4

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