不同产区麦冬、山麦冬rDNA-ITS序列分析

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基金项目:

国家科技支撑计划项目(2006BAI06A11-03);福建省农业科学院青年科技人才创新基金项目(A2007QJ02)

rDNA-ITS of Radix ophiopogonis and Radix liriopes from different regions

1.
Institute of Agrobiological Resources, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;
2.
Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

摘要: 利用特异性引物进行PCR扩增和克隆、测定不同产区麦冬和山麦冬原植物的18S～26S r DNA-ITS区碱基序列,结果得到供试的7个不同产区麦冬和山麦冬植株样品rDNA的ITS及5.8SrDNA全序列、18S和26SrDNA部分序列,序列长度在696～711 bp。通过分析比较各ITS序列,表明不同产区麦冬和山麦冬rDNA-ITS区存在差异,可为其分子鉴别提供了依据。
- 麦冬 /
- 山麦冬 /
- rDNA-ITS
Abstract: The 18S-26S rDNA-ITS base sequences of Radix ophiopogonis and Radix liriopes from different geographical regions were compared by using the specific PCR-ITS.The complete sequences of ITS and 5.8S rDNA,as well as partial sequences of 18S rDNA and 26S rDNA,in the range of 696-711 bp for the seven samples were obtained.By comparing the sequences,it was apparent that the ITS sequences of R. ophiopogonis and R. liriopes from different regions varied.Our analytical results provided a basis for molecular identification of the species.
- Radix ophiopogonis /
- Radix liriopes /
- rDNA-ITS

[1]	余永邦,秦民坚,梁之桃,等.不同产区太子参的rDNA ITS区序列的比较[J].植物资源与环境学报,2003,12(4):1-5.
[2]	LEE S B,RASMUSSEN S K.Molecular markers in some medicinal plants of the Apiaceae family[J].Euphytica,2000,114:87-91.

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