黄曲条跳甲对敌敌畏抗性机理的初步研究

郑丽祯; 傅建炜; 曹慧芳; 李建宇; 占志雄

黄曲条跳甲对敌敌畏抗性机理的初步研究

1.
福建省农业科学院植物保护研究所, 福建, 福州, 350013

基金项目:

福建省自然科学基金(B0610025)

详细信息

通讯作者:
占志雄(1964- ),男,研究员,主要从事农业害虫综合治理和杀虫剂的研究(E-mail:

中图分类号: S346
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出版历程
- 收稿日期: 2009-10-12
- 修回日期: 2009-12-01
- 刊出日期: 2009-12-15

Mechanism of dichlorvos-resistance of striped flea beetle, Phyllotreta striolata(Coleoptera:Chrysomelidae)

1.
Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

摘要

摘要: 采用敌敌畏进行连续2次的黄曲条跳甲种群汰选,以获得中高抗个体,并测定汰选种群和对照种群的药剂敏感性,以及羧酸酯酶、乙酰胆碱酯酶和谷胱甘肽-S-转移酶的比活力及动力学常数。结果表明敌敌畏汰选种群的LC50略高于对照种群,羧酸酯酶的最大反应速度Vmax为0.0081,米氏常数Km为0.6179,均明显小于对照种群的最大反应速度(Vmax=0.0849)和米氏常数(Km=2.3699);敌敌畏汰选种群的乙酰胆碱酯酶的比活力为0.0074 OD412insect-1min-1,低于对照种群的乙酰胆碱酯酶比活力(0.0096 OD412insect-1min-1),且差异显著;敌敌畏汰选种群的谷胱甘肽-S-转移酶酶比活力为0.0050 OD340insect-1min-1,高于对照种群的谷胱甘肽-S-转移酶酶比活力(0.0036 OD340insect-1min-1),二者差异显著。说明黄曲条跳甲的抗药性与羧酸酯酶、谷胱甘肽-S-转移酶的活性提高以及乙酰胆碱酯酶活性的降低有关。
- 黄曲条跳甲 /
- 敌敌畏 /
- 抗性机理
Abstract: Conventional insecticide control became less effective and the beetle's insecticide resistance increased.This study screened the SFB populations by exposing the insects to dichlorvos two consecutive times to obtain high resistance individuals.The insecticide susceptibility,activities of carboxylesterase(CarE),acetylcholinesterase(AChE) and glutathione-S-transferase(GST),as well as the kinetic constants of screened(SP) and control populations(CP) were determined.The results indicated that LC50 value of SP was slightly higher than CP.The maximum velocity(Vmax) and Michaelis constant(Km) of CarE in SP were much less than that in CP.The AchE activity of SP was 0.0074 OD412insect-1min-1,significantly lower than that of CP(0.009 6 OD412 insect-1min-1).And,the GST activity of SP was 0.0050 OD340insect-1min-1,significantly higher than that of CP(0.0036 OD340insect-1min-1).Our study suggested that the resistance of SFB to dichlorvos might relate to the decreased activity of AChE as well as the increased activities of CarE and GST.
- Phyllotreta striolata /
- dichlorvos /
- resistance mechanism

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参考文献(9)

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