摘要:
采用PCR和RT-PCR技术,对福建省1个规模化猪场的仔猪腹泻样本进行致病性大肠杆菌(EPEC)、猪传染性胃肠炎病毒(TGEV)、猪流行性腹泻病毒(PEDV)和猪轮状病毒(RDV)的感染情况调查.结果表明:15个猪场EPEC检测为阴性,1个猪场同时检测到大肠杆菌K88的201 bp条带和仔猪水肿病大肠杆菌F18ab 菌毛蛋白的510bp条带,EPEC的阳性率为6.25%. 1个猪场检测到TGEV的497bp特有条带,TGEV的阳性率为6.25%.3个猪场检测到PEDV的854 bp特有条带,PEDV的阳性率为18.75%. 3个猪场检测到RDV的342 bp特有条带,RDV的阳性率为18.75%.1个猪场同时感染EPEC和PEDV 2种病原,其余的15个猪场均为单一病原感染.表明福建省规模化猪场的仔猪腹泻仍然存在EPEC、TGEV、PEDV、RDV病原感染,呈现散发流行,与以往的报道相比阳性率明显下降.
Abstract:
PCR and RT-PCR were used to investigate the infection caused by enteropathogenic Escherichia coli(EPEC),transmissible gastroenteritis virus of swine(TGEV),porcine epidemic diarrhea virus of swine(PEDV) and rotavirus of swine(RDV) in piglets from 16 large-scale swine farms in Fujian in 2009.The result showed that only one farm was infected with the 201bp band of E.coli K88 and the 510bp band of F18ab fimbriae of E.coli eliciting edema disease of piglet.EPEC was not found in other farms.The positive rate of EPEC was 6.25%.There was a farm,in which the 497bp band of TGEV was found with a positive rate of 6.25%.Three farms were found with the 854bp band of PEDV or 342bp band of RDV.Hence,the positive rates of TGEV and RDV were both 18.75%.It was concluded that the EPEC,TGEV,PEDV and RDV diarrhea infection continued to exist in piglets in the large-scale swine farms in Fujian.However,the infection showed up only in separate areas,and the rates were significantly reduced as compared to the previous reports.
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