摘要:
应用RT-PCR方法分段扩增DHV-NA株cDNA,并进行基因组全序列测定及同源性等分析.结果显示DHV-NA株基因组全长7 692 bp(不包括PolyA),＇和3＇非编码区长度分别为626 bp和316 bp,有1个单一开放阅读框架(ORF,627～7 376 nt),编码2 249个氨基酸;与I型DHV(DHV-Ⅰ)参考株比较,其核苷酸和氨基酸同源性分别为94.3%～96.9%和97.8%～98.8%;与新型DHV(N-DHV)90D株比较,其核苷酸和氨基酸同源性分别为72.6%和82.3%.表明NA株与DHV-Ⅰ参考株遗传进化关系较密切,而与N-DHV遗传关系较远.
Abstract:
The cDNA fragments of DHV-NA were amplified with RT-PCR.Complete genomic sequence was determined and phylogenetic tree was analyzed.The complete genome of DHV-NA was determined to consist of 7692 nucleotides,except Poly(A) tail.The lengths of 5′and 3′uncoding regions were 626 and 316 nucleotides,respectively.There was a single open reading frame(ORF,627-7376nt) encoding 2249 amino acids.Compared to the other DHV-Ⅰstrains(i.e.,R85952,H,DRL-62,C80,03D,JX),the nucleotide sequence homology was 94.3%-96.9% and the amino acids sequence homology was 97.8%-98.8%.Compared to NDHV-90D strain,the homologies of the nucleotide sequence and amino acid sequence were 72.6% and 82.3%,respectively.The results indicated that the phylogenetic homology between DHV-NA and other DHV-Ⅰstrains was higher than that between DHV-NA and N-DHV 90D strain.
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