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抗、感青枯病桑树根际细菌群落结构与多样性

武华周 娄德钊 涂娜娜 耿涛 卢芙萍 吉训聪 王树昌

武华周,娄德钊,涂娜娜,等. 抗、感青枯病桑树根际细菌群落结构与多样性 [J]. 福建农业学报,2020,35(9):1004−1011 doi: 10.19303/j.issn.1008-0384.2020.09.012
引用本文: 武华周,娄德钊,涂娜娜,等. 抗、感青枯病桑树根际细菌群落结构与多样性 [J]. 福建农业学报,2020,35(9):1004−1011 doi: 10.19303/j.issn.1008-0384.2020.09.012
WU H Z, LOU D Z, TU N N, et al. Rhizosphere Bacterial Community and Diversity at Fields of Wilt Resistant or Susceptible Mulberry Trees [J]. Fujian Journal of Agricultural Sciences,2020,35(9):1004−1011 doi: 10.19303/j.issn.1008-0384.2020.09.012
Citation: WU H Z, LOU D Z, TU N N, et al. Rhizosphere Bacterial Community and Diversity at Fields of Wilt Resistant or Susceptible Mulberry Trees [J]. Fujian Journal of Agricultural Sciences,2020,35(9):1004−1011 doi: 10.19303/j.issn.1008-0384.2020.09.012

抗、感青枯病桑树根际细菌群落结构与多样性

doi: 10.19303/j.issn.1008-0384.2020.09.012
基金项目: 国家现代农业产业技术体系专项(CARS-18-SYZ17);中国热带农业科学院基本科研业务费专项(1630042019021);中央引导地方科技发展专项(ZY2018HN01)
详细信息
    作者简介:

    武华周(1987−),男,硕士,助理研究员,研究方向:桑树栽培与育种(E-mail:wuhuazhouaa@126.com

    通讯作者:

    王树昌(1976−),男,博士,研究员,研究方向:热带桑蚕技术研究(E-mail:wangshuchang2001@163.com

  • 中图分类号: S 432

Rhizosphere Bacterial Community and Diversity at Fields of Wilt Resistant or Susceptible Mulberry Trees

  • 摘要:   目的  通过抗、感青枯病桑树根际土壤细菌群落结构与多样性分析,阐明根际细菌群落与桑树抗、感青枯病的相关性。  方法  采用Illumina MiSeq测序技术对抗青枯病桑树基因型(抗青283×抗青10)和感病基因型(桂桑优62)根际细菌的16S rRNA基因V3-V4区进行扩增,高通量测序后比较分析了其根际细菌群落结构与多样性。  结果  ① 抗、感青枯病桑树根际土壤的优势菌门为变形菌门、放线菌门、酸杆菌门、芽单胞菌门和拟杆菌门,优势菌属为MND1Gaiella、硝化螺菌属、Haliangium和链霉菌属;② 抗、感青枯病桑树根际土壤细菌群落Alpha多样性无显著差异,但经NMDS排序,则可显著区分(stress=0.005<0.05),其中抗性基因型根际土壤中具有重要作用的细菌类群较多,包括:硝化螺旋菌纲、酸杆菌纲、硝化螺旋菌目、索利氏菌目、酸杆菌目、硝化螺旋菌科、酸杆菌目未培养科、索利氏菌科Subgroup 3和Subgroup 2未培养科,而敏感基因型桂桑优62根际重要作用的细菌类群则主要为Ilumatobacteraceae和TRA3 20 Other两个科。③ 抗、感青枯病桑树根际细菌差异COG类目为763,仅占17.25%。  结论  抗、感青枯病桑树根际土壤细菌群落丰富度和多样性虽无显著差异,但其重要作用的类群显著不同,说明桑树根际土壤细菌群落结构可能与种质对青枯病的抗性具有相关性。本结果将为进一步研究桑树根际微生态特征、筛选有益功能菌株并用于桑青枯病的有效防控具有重要意义。
  • 图  1  抗感青枯病桑树根际土壤细菌OTUs韦恩图

    Figure  1.  Venn diagram of bacteria OTUs in rhizosphere soils at QZ2K and QZ2G

    图  2  细菌群落门(A)和属(B)水平相对丰度

    Figure  2.  Phylum (A) and genus (B) distributions of OTUs

    图  3  抗感青枯病桑树根际土壤细菌NMDS分析

    Figure  3.  Non-MetricMulti-dimensional scaling by UUF metric

    图  4  抗感青枯病桑树根际土壤细菌LEfSe分析

    Figure  4.  LEfSe analysis on bacteria in rhizosphere soils at QZ2K and QZ2G

    图  5  抗感青枯病桑树根际细菌预测得到的COG相对丰度

    注:A: RNA加工与修饰; B: 染色体结构与动态;C: 能量产生与转化; D: 细胞周期控制,细胞分裂,染色体分裂; E: 氨基酸转运与代谢; F: 核酸转运与代谢;G:碳水化合物的运输与代谢; H: 酶运输与代谢; I: 脂质运输与代谢; J:翻译,核糖体结构和生物发生; K: 转录; L: 复制,重组和修复; M: 细胞壁/膜/包膜生物发生; N: 细胞运动; O: 翻译后修饰,蛋白质更新,伴侣; P: 无机离子的运输与代谢; Q:次生代谢产物的生物合成,转运和分解代谢; R:仅通用功能预测; S:未知功能; T:信号转导机制; U:细胞内运输,分泌和囊泡运输; V: 防御机制; W: 细胞外结构; Z: 细胞骨架。

    Figure  5.  Relative abundance of COG at QZ2K and QZ2G

    Note: A: RNA processing and modification; B: Chromatin structure and dynamics; C: Energy production and conversion; D: Cell cycle control, cell division, and chromosome partitioning; E: Amino acid transport and metabolism; F: Nucleotide transport and metabolism; G: Carbohydrate transport and metabolism; H: Coenzyme transport and metabolism; I: Lipid transport and metabolism; J: Translation, ribosomal structure and biogenesis; K: Transcription; L: Replication, recombination, and repair; M: Cell wall/membrane/envelope biogenesis; N: Cell motility; O: Posttranslational modification, protein turnover, and chaperones; P: Inorganic ion transport and metabolism; Q: Secondary metabolites biosynthesis, transport, and catabolism; R: General function prediction only; S: Function unknown; T: Signal transduction mechanisms; U: Intracellular trafficking, secretion, and vesicular transport; V: Defense mechanisms; W: Extracellular structures; Z: Cytoskeleton.

    表  1  高通量测序数据概况

    Table  1.   Overview of high-throughput sequencing data

    样品
    Sample
    高质量序列
    Clean tags
    优质序列
    Valid tags
    平均长度 /bp
    Valid mean length
    测序深度指数/%
    Goods coverage
    QZ2K.133 88328 94842994.81
    QZ2K.238 78632 37143094.69
    QZ2K.332 32826 94143294.57
    QZ2K.441 49934 26743294.56
    平均值 average36 624.030 631.8430.894.658
    QZ2G.138 74732 49643294.53
    QZ2G.237 62631 68843294.43
    QZ2G.340 749 34 81443294.78
    QZ2G.440 65634 09043295.22
    平均值 average3 944.533 272.0432.094.740
    下载: 导出CSV

    表  2  抗感青枯病桑树根际土壤细菌α多样性指数(平均值±标准误,n=4)

    Table  2.   Alpha diversity indices of bacteria in rhizosphere soils at QZ2K and QZ2G(Mean±SE,n=4)

    样品
    Samples
    谱系多样性指数
    PD whole Tree
    Chao1文库覆盖率(%)
    Goods coverage
    观测物种数
    Observed Species
    香浓-威纳指数
    Shannon-Wiener
    辛普森指数
    Simpson
    QZ2K84.97±0.773 190.57±22.480.95±0.002 333.88±40.499.51±0.090.995±0.001
    QZ2G82.86±1.443 178.61±87.450.95±0.002 304.30±49.589.65±0.030.997±0.001
    下载: 导出CSV
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  • 收稿日期:  2020-08-10
  • 修回日期:  2020-09-08
  • 刊出日期:  2020-09-28

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