短葶山麦冬遗传多样性的ISSR标记研究

基金项目:

福建省科技计划项目(2009N0041);福建省自然科学基金项目(2009J05078);泉州市科技计划重点项目(2008N40)

Genetic diversity of Liriope muscari(Decn.)Bailey detected by using ISSR markers

1.
Department of Bioengineering & Biotechnology, Huaqiao University, Xiamen, Fujian 361021, China

摘要: 利用ISSR分子标记对47种短葶山麦冬种质资源进行遗传多样性分析,18条引物共扩增出131个位点,多态性位点为83个,占63.4%.通过UPGMA聚类分析,47份短葶山麦冬种质分为四大类,遗传相似系数(GS)变化范围在0.64~0.98之间,其中莆田仙游的野生品种为一类,与地方品种间遗传差异最大.聚类分析的结果显示短葶山麦冬的亲缘关系与地理分布的相关性不显著.
- 简单序列重复区间扩增多态性 /
- 短葶山麦冬 /
- 遗传多样性
Abstract: ISSR markers were applied to detect the genetic diversities of 47 cultivars of Liriope muscari(Decn.)Bailey.One hundred thirty one bands were amplified with 18 primers,including 83 polymorphic bands.The percentage of the polymorphic bands was 63.4%.By cluster analysis based on ISSR markers using UPGMA,the 47 cultivars were classified into 4 groups.The genetic similarity was from 0.64 to 0.98.As a group,the wild species from Putian had the greatest genetic difference from the landrace.Determined by the cluster analysis,the geographical distribution did not seem to be distinctive.The genetic diversity of L. muscari Bailey appeared to be high,based on our observation.This could provide references for analyzing genetic relationship and utilizing the genetic diversity for agricultural applications.
- inter-simple sequence repeat /
- Liriope muscari(Decne.)Bailey /
- genetic diversity

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