鸡J亚型白血病病毒荧光定量PT-PCR检测方法的建立

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Quantitative detection of ALV-J by fluorescence RT-PCR

1.
Institute of Animal Husbandry & Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

摘要: 运用实时荧光定量PCR技术,针对鸡J亚型白血病的gp85基因设计特异性引物,建立了一种快速准确诊断鸡J亚型白血病病毒(ALV-J)的荧光定量PT-PCR方法.通过重复性、特异性和灵敏度等验证表明,该方法适用于ALV-J的临床检测,为ALV-J引起的鸡肿瘤性疾病的快速诊断及采取相应的治疗措施提供了重要的技术支撑.
- 鸡白血病 /
- 荧光定量PT-PCR /
- gp85基因
Abstract: Avian leukosis is one of the main chicken tumor diseases.It causes considerable economic loss for poultry farmers.A new,rapid diagnostic mehtod applying the real-time florescence quantitative polymerase chain reaction(PT-PCR)was developed for detecting the avian leukosis virus subgroup J(ALV-J).A set of subgroup J special primers for gp85 gene was used.Validations on repeatability,specificity and sensitivity of the methodology showed its potential applicability for clinical diagnosis of ALV-J.
- avian leukosis /
- RT-PCR /
- gp85 gene

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