摘要:
为探讨子宫内膜炎母猪子宫内细菌感染的情况及其致病性,试验采用常规细菌分离培养、ATB自动细菌鉴定及ATB自动药敏测试法,对40头子宫内膜炎淘汰母猪和15头无子宫内膜炎母猪的子宫体和子宫角样品进行细菌分离和鉴定。结果从40头子宫内膜炎母猪样品共分离到70株细菌,分离率100%,其中从子宫体共分离到47株,子宫角分离到23株;从15头无子宫内膜炎母猪中1份子宫体样品分离到2株菌,其余均未分离到细菌,分离率6.67%。72株分离菌,经鉴定主要为大肠杆菌、铜绿假单胞菌、恶臭假单胞菌、嗜水气单胞菌等16种。分离菌中铜绿假单胞菌、嗜水气单胞菌对小白鼠有较强致病力,并对28种抗生素都有不同程度的耐药性。结果表明,生产母猪高淘汰率与母猪子宫内膜炎发生率有关;环境中常在的条件性致病菌是母猪子宫内膜炎的主要感染菌,并对多种抗生素有耐药性。
关键词:
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母猪 /
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子宫内膜炎 /
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细菌 /
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药敏试验
Abstract:
40 sows with endometritis and 15 sows with non-endometritis were selected and butchered in slaughter house.Mucus samples of uterine horn and uterine cervix were collected from the sows and inoculated into the culture medium.All the bacteria were purified and identified by automated bacterial identification and antibiotics susceptibility test system(ATB).Pathogenicity of each identified strains were determined by challenge intra-peritoneally to white mouse.The results show that from 40 sows with endometritis,70 isolates were determined,among them,47 isolates were in uterine cervix and 23 isolates were in uterine horn respectively.The positive rate was 100%.2 isolates were determined in uterine cervix of one of 15 sows with non-endometritis.The positive rate was 6.67%.Among the 72 isolates,16 strains were identified.The main strains were Escherichia coli,Pseudomonas aeruginosa,Pseudomonas putida and A.hydrophila and only Pseudomonas aeruginosa and A.hydrophila showed pathogenic effect in white mice.Antibiotics susceptibility test on the isolated strains of P.aeruginosa and A.hydrophila exhibited that they had strong resistance to 28 antibiotics.The results indicated that high culling rate of sows are related to endometritis.Environmental bacteria are main pathogenic bacteria of endotremitis conditionally,which have resistance to antibiotics mainly used in clinical practice.
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