福建省大豆根腐病病原菌的鉴定

李本金; 陈庆河; 兰成忠; 王娜娜; 王源超; 翁启勇

福建省大豆根腐病病原菌的鉴定

1.
福建省农业科学院植物保护研究所,福建福州 350013;
2.
南京农业大学植物病理学系,江苏南京 210095

基金项目:

国家大豆产业技术体系项目(Nycytx-004);福建省农科教项目([2010]627号);福建省自然科学基金(2011J01116);福建省财政专项福建省农业科学院科技创新团队建设基金(STIF-Y07)

详细信息

作者简介:
李本金(1969-),女,副研究员,主要从事植物病理及分子生物学研究(E-mail:lbenjin@126.com)

通讯作者:
翁启勇(1962-),男,研究员,主要从事作物有害生物综合治理技术研究和应用(E-mail:wengqy@faas.cn)

中图分类号: S 565.1
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出版历程
- 收稿日期: 2011-08-14
- 修回日期: 2011-09-17
- 刊出日期: 2011-10-15

Identification and Pathogenicity Test of the Pathogens Causing Soybean Root Rot in Fujian

1.
Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
2.
Department of Plant Pathology, Nanjing Agricultural University, Nanjing,Jiangsu 210095, China

摘要

摘要: 为明确福建省大豆根腐病病原菌的种类及组成,于2007~2009年从漳州、厦门、龙岩等地大豆产区采集根腐病标样216份,从中分离到307株丝状病原真菌。根据其培养性状、形态学特征、代表性菌株的致病性测定及rDNA-ITS序列分析,将这些病原菌分为5种,分别为尖孢镰刀菌(Fusarium oxysporum,112株)、茄腐镰刀菌(Fusarium solani,74株)、大豆疫霉菌(Phytophthora sojae,79株)、立枯丝核菌(Rhizoctonia solani,24株)和瓜果腐霉菌(Pythium aphanidermatum,18株)。致病性测定结果表明,5种病原菌的致病力存在明显差异,其中大豆疫霉菌、尖孢镰刀菌和茄腐镰刀菌的致病性较强,发病率分别为100%、97.8%和95.6%,立枯丝核菌、瓜果腐霉菌则相对较弱。
- 大豆根腐病 /
- 病原鉴定 /
- ITS /
- 致病性
Abstract: Two hundred and sixteen soybean root rot samples were collected from Zhangzhou, Xiamen, Longyan city in Fujian province during 2007 to 2009 in order to determine the fungal pathogens that could cause soybean root rot, and three hundred and seven filamentous fungi strains were isolated from the samples. Five groups of pathogen were identified, which were Fusarium oxysporum (112 isolates), Fusarium solani (74 isolates), Phytophthora sojae (79 isolates), Rhizoctonia solani (24 isolates),Pythium aphanidermatum (18 isolates) according to the cultural characteristics, morphology, pathogenicity and the rDNA-ITS sequences of the isolates. The results of the pathogenicity of these pathogens revealed that P. sojae, F. oxysporum and F. solani isolates showed strong pathogenicity to soybean roots by artifical infection, leading to disease incidences by 100%、97.8% and 95.6%, respectively, but R. solani and P. aphanidermatum isolates showed weak pathogenicity.
- soybean root rot /
- pathogen identification /
- ITS /
- pathogenicity

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参考文献(7)

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