青枯雷尔氏菌致病机制及其相关基因的研究进展

林海云; 车建美; 刘波; 郑雪芳; 唐唯其; 朱育菁

青枯雷尔氏菌致病机制及其相关基因的研究进展

1.
福建省农业科学院农业生物资源研究所,福建福州 350003;
2.
福建农林大学生物农药与化学生物学教育部重点实验室,福建福州 350002

基金项目:

国家自然科学基金项目(30871667);福建省自然科学基金项目(2008J0054,2009J01087);福建省财政专项福建省农业科学院科技创新团队建设基金(STIF-Y03)

详细信息

作者简介:
林海云(1988-),女,硕士研究生,研究方向为生物农药(E-mail:linhaiyun880518@163.com)

通讯作者:
刘波(1957-),男,博士,研究员,研究方向为微生物生物技术与农业生物药物(E-mail:liubofaas@163.com)

中图分类号: S 436.412
计量
- 文章访问数: 4669
- HTML全文浏览量: 124
- PDF下载量: 2151
- 被引次数: 0
出版历程
- 收稿日期: 2011-07-24
- 修回日期: 2011-08-25
- 刊出日期: 2011-10-15

Advances research in Ralstonia solanacearum Pathogenicity Mechanism and Related Genes

1.
Agricultural Bio-resources Resource Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou, Fujian 350003,China;
2.
Key Laboratory of Biopesticide and Chemical Biology,Ministry of Education,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China

摘要

摘要: 阐述青枯雷尔氏菌致病基因以及它们之间的相互调节。由于青枯雷尔氏菌的复杂性,进而发展了许多青枯雷尔氏菌分子鉴定技术,并且对青枯雷尔氏菌的鉴定逐渐走向快速、便捷和灵敏高的趋势。青枯雷尔氏菌基因组约5.8 Mb,具有高(G+C)含量和约5 120个可能的编码基因;它是由3.7 Mb的染色体和2.1 Mb的大质粒所组成,主要的致病因子有Ⅲ型hrp分泌系统产物、胞外多糖、细胞壁降解酶(包括果胶质酶以及纤维素酶等),其涉及的基因主要包括hrp基因簇、avr基因、毒性基因;青枯雷尔氏菌通过Ⅲ型分泌系统(T3SS)、II型分泌系统(T2SS)等分泌系统将多种毒性因子输送到胞外使寄主植物致病。同时,T3SS和T2SS之间也是相互影响的。上述致病因子的协调作用是由一个复杂的网络调节系统控制的,并以PhcA调节基因的启动和转录为核心,自动而精密地调节有关致病基因的表达及关闭,从而控制细菌的生长状态。
- 青枯雷尔氏菌 /
- 菌株鉴定 /
- 致病因子 /
- 致病基因 /
- 致病机制
Abstract: This review was focused on the virulent and avirulent genes and the regulation of Ralstonia solanacearum pathogenicity. Ralstonia solanacearum had been described as a species-complex bacterium, therefore, many technology were applied to identify this bacterium. Meanwhile, the identification of R. solanacearum became more and more quick, convenient and sensitive. The genome of R.solanacearum strain had a size of 5.8 Mb with a high (G+C) content and a coding potential for approximately 5 120 proteins. It was organized in two replicons, a 3.7 Mb chromosome and a 2.1 Mb megaplasmid. The products of type III hrp section system, extrapolysaccharide, cell wall degrading enzymes such as pectinolytic and cellalolytic enzymes constituted were the major factors for the pathogenicity. All of these were mainly related to the hrp gene cluster, avr gene and virulence genes. The Type III secretion system (T3SS) and Type II secretion system (T2SS) that directly translocated effector proteins into the host cells were essential for the development of disease. Moreover, T2SS functionally interacts with T3SS. The virulence and pathogenicity genes of R. solanacearum were controlled by an elaborate sensory network. The system used PhcA concentration as a core to regulate the expression of pathogenic factors, and thus the growth status of bacteria.
- Ralstonia solanacearum /
- strains identification /
- virulence factors /
- pathogenicity genes /
- pathogenicity mechanism

HTML全文

参考文献(23)

[1]
[2]	JUNG K M,LEE M H,SHIM J K,et al. PCR-based specific detection of Ralstonia solanacearum by amplification of Cytochrome c1 signal peptide sequences[J]. Journal of Microbiology and Biotechnology,2007,17(11):1765-1711.
[3]	KUBOTA R,VINE B G,ALVAREZ A M,et al. Detection of Ralstonia solanacearum by loop-mediated isothermal amplification[J]. Phytopathology,2008,98(9):1045-1051.
[4]
[5]	FOUCHE W J,POUSSIER S,TRIGALET D D,et al. Molecular identification of some African strains of Ralstonia solanacearum from eucalypt and potato[J]. Journal of General Plant Pathology,2006,72(6):369-373.
[6]
[7]	赖世龙,侯浩,姜伟. 细菌的运动性及其在致病初期过程中的作用[J]. 微生物学杂志,2006,26(5):68-70.
[8]
[9]
[10]	ARLART M,GOUGH C L,ZISCHEK C,et al.Tran-scriptional organization and expression of the large hrp gene cluster of Pseudomonas solanacearum[J]. Molecular Plant-Microbe Interactions,1992,5(2):187-193.
[11]	MUKAIHARA T,TAMURA N,MURATA Y,et al. Genteic screening of Hrp type III-related pathogenicity genes controlled by the HrpB transcriptional activator in Ralstonia solanacearum[J].Molecular Microblology,2004,54(4):863-875.
[12]	杨军,尹启生,宋纪真,等. 植物病原细菌的hrp基因[J]. 遗传,2005,27(5):852-858.
[13]	BUTTNER D,HE S Y. Type III protein secretion in plant pathogenic bacteria[J]. Plant Physiology,2009,150(4):1656-1664.
[14]
[15]	刘焕利,何礼远,毛国璋,等. 植物青枯细菌胞外蛋白在致病中作用的研究[J],中国农业科学,2000,33(1):57-61.
[16]
[17]
[18]
[19]	GALAN J E,CLLMER A. Type III secretion machines: bacterial devices for protein delivery into host cells[J]. Science,1999,284(5418):1322-1328.
[20]	BRITO B,MARENDA M,BARBERIS P,et al. prhJ and hrpG,two new components of the plant signal-dependent regulatory cascade controlled by PrhA in Ralstonia solanacearum[J]. Molecular Microbiology,1999,31(1):237-251.
[21]
[22]	YOSHIMOCHI T,HIKICHI Y,KIBA A,et al. The global virulence regulator PhcA negatively controls the Ralstonia solanacearum hrp regulatory cascade by repressing expression of the PrhIR signaling proteins[J]. Journal of Bacteriology,2009,191(10):3424-3428.
[23]	HIKICHI Y,YOSHIMOCHI T,TSUJIMOTO S,et al. Global regulation of pathogenicity mechanism of Ralstonia solanacearum[J]. Plant Biotechnology,2007,24(1):149-154.