香蕉枯萎病芽胞杆菌生防菌的筛选及其抑菌特性研究

林营志; 刘国红; 刘波; 肖荣凤

香蕉枯萎病芽胞杆菌生防菌的筛选及其抑菌特性研究

1.
福建省农业科学院,福建福州 350003;
2.
福建农林大学,福建福州 350002;
3.
福建省农业生物药物工程技术研究中心,福建福州 350003

基金项目:

国家948项目(2011-G25);农业部科技专项子课题(200903049-08);福建省科技计划项目省属公益类科研院所基本科研专项(2009R10037-1)

详细信息

作者简介:
刘波(1957-),男,研究员,博士,研究方向:微生物生物技术与农业生物药物(E-mail:fzliubo@163.com)

中图分类号: R 378.7
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出版历程
- 收稿日期: 2011-08-22
- 修回日期: 2011-09-23
- 刊出日期: 2011-12-15

Screening Biological Control Agents, Bacillus spp., Against Fusarium oxyspomm f sp. Cubense and its Inhibitory Characteristics

1.
Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China;
2.
Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China;
3.
Reseach Centre of Agricultural Biological Chemincal Engineering of Fujian, Fuzhou, Fujian 350003, China

摘要

摘要: 从我国不同地区采集土样,共分离到300余株芽胞杆菌,通过平板对峙生长法从中筛选到4株对香蕉枯萎病原菌有明显拮抗作用的菌株。经16S rRNA 序列分析,将这4株生防芽胞杆菌鉴定为多粘类芽胞杆菌。4株菌的生物学特性基本一致,其主要脂肪酸类型为15∶0 ANTEISO,分析发现生防菌对病原菌的抑菌作用与生防菌的脂肪酸类型具有相关性。从菌量、温度、pH以及紫外照射时间对4株生防菌的抑菌特性进行了研究,结果表明:4株菌的抑菌效果随着浓度的降低而降低,当菌液稀释100倍时只有FJAT-4539具有较强的抑菌作用;4株菌的抑菌作用对温度敏感,加热会导致抑菌活性丧失;4株菌均在pH为9时对病原菌具有强的抑菌作用;4株菌对紫外耐受力强。综合所有试验结果,菌株FJAT-4539对香蕉枯萎病的抑菌效果最强。
- 芽胞杆菌 /
- 香蕉枯萎病原菌 /
- 抑菌作用
Abstract: More than three hundred Bacillus spp.were isolated from the soil samples in China, and there were four strains that had effective in suppression Fusarium oxyspomm f sp.cubense on PDA agar plates.These four strains were identified as Paenibacillus polymyxa based on 16S rRNA sequence.There were similar biological characterics, and main fatty acid type was 15∶0 ANTEISO among those strains.There are correlation between the inhibitory effect and some fatty acid based on DPS soft analysis.The characterization, such as different account, temperature, pH and UV time, were studied,and the effect of 4 Paenibacillus polymyxa against Fusarium oxyspomm f sp.cubense were also studied in this study.The results showed that the effect of four Bacillus species were decreased by concentration, and sensitive to temperature.And four strains had strong inhibitory effect at pH9, and not sensitive to UV.The results showed that that strain FJAT-4539 was the strongest strain to inhibit banana wilt disease among the four Bacillus spp.
- Biological Control Bacillus spp. /
- Banana wilt disease /
- inhibitory effect

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参考文献(8)

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