粳稻云引受稻瘟病菌诱导表达基因的荧光定量分析

王颖姮; 朱永生; 蔡秋华; 廖长见; 谢鸿光; 吴方喜; 张建福; 谢华安

粳稻云引受稻瘟病菌诱导表达基因的荧光定量分析

王颖姮^1,2,3,4,
朱永生^1,2,3,4,
蔡秋华^1,2,3,4,
廖长见⁵,
谢鸿光^1,2,3,4,
吴方喜^1,2,3,4,
张建福^1,2,3,4,,
谢华安^1,2,3,4,

1.
福建省农业科学院水稻研究所,福建福州 350018;
2.
福州国家水稻改良分中心/农业部华南杂交水稻种质创新与分子育种重点实验室/福建省作物分子育种工程实验室/福建省水稻分子育种重点实验室,福建福州 350003;
3.
福建省作物种质创新与分子育种省部共建国家重点实验室培育基地,福建福州 350003;
4.
水稻国家工程实验室,福建福州 350003;
5.
福建省农业科学院作物研究所,福建福州 350013

基金项目:

国家"863"计划项目(2006AA100101、2010AA101804、2010AA101801);国家自然科学基金项目(30891509);福建省自然科学基金重点项目(2007J0005);福建省杰出青年基金项目(2009J06011);福建省自然科学基金项目(2010J01103、2010J01102);福建省财政专项——福建省农业科学院创新团队项目(STIF-Y04)

详细信息

通讯作者:
张建福(1971-),男,博士,副研究员,主要从事水稻遗传学与分子育种研究
谢华安(1941-),男,研究员,中国科学院院士,主要从事水稻遗传育种研究

张建福(1971-),男,博士,副研究员,主要从事水稻遗传学与分子育种研究
谢华安(1941-),男,研究员,中国科学院院士,主要从事水稻遗传育种研究

中图分类号: S 511
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出版历程
- 收稿日期: 2011-11-24
- 修回日期: 2011-12-16
- 刊出日期: 2012-01-30

Real-time PCR Analysis of Genes Induced with the Magnaporthe oryzae from Japonica Rice Yunyin

WANG Ying-heng^1,2,3,4,
ZHU Yong-sheng^1,2,3,4,
CAI Qiu-hua^1,2,3,4,
LIAO Chang-jian⁵,
XIE Hong-guang^1,2,3,4,
WU Fang-xi^1,2,3,4,
ZHANG Jian-fu^{1,2,3,4
,},
XIE Hua-an^{1,2,3,4
,}

1.
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350018,China;
2.
Fuzhou Branch, National Rice Improvement Center of China/Key Laboratory of Hybrid Rice Germplasm Innovation and Molecular Breeding of South China, Ministry of Agriculture, P.R.China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding, Fuzhou, Fujian 350003,China;
3.
Incubator of National key Laboratory of Fujian Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Sciences & Technology,P.R.China,Fuzhou,Fujian 350003,China;
4.
National Engineering Laboratory of Rice of China, Fuzhou, Fujian 350003,China;
5.
Crop Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China

摘要

摘要: 前期通过对广谱稻瘟病抗性品种粳稻云引接种稻瘟病菌后的基因表达谱分析,构建随时间变化的动态样本基因调控网络。在此基础上,选取该调控网络中10个比较重要的基因,采用Real-Time PCR的方法对其在接种稻瘟病菌后的时空表达进行相对定量分析,得到这些基因在稻瘟病菌诱导后在云引中的时空表达模式。10个基因均受到稻瘟病菌的诱导,但达到表达量峰值的时间各异,有8个基因在36 h内表达量达到峰值,3个编码转录因子的基因表达量在24 h内达到峰值;部分基因表达呈先上升,后抑制,另一些基因的表达则先抑制,后上升,再下降。该研究为进一步确定这些基因在云引抗病性调控中的作用奠定基础。
- 云引 /
- 稻瘟病 /
- 实时荧光定量PCR /
- 基因表达及调控
Abstract: In early stage, we analysed the gene expression profile of broad-spectrum rice blast resistance japonica variety Yunyin induced by Mangnaporthe oryzae, and constructed the gene co-expression network based on the dynamic samples. Ten important genes based on this network were chosen in this study, and the expression pattern of 10 genes for japonica rice Yunyin inoculated with Mangnaporthe grisea was analyzed. The highest expression stages of the 10 genes were different, of which 8 genes reached the highest expression stage at 36 hours, and 3 genes encoding transcription factors reached the highest expression stage at 24 hours. Meanwhile, some of the 10 genes expression were up-regulated at first, and then inhibited. Others were inhibited at first, then increased, and declined at last. This work will help us further to define the role of japonica rice Yunyin in disease resistance.
- Yunyin /
- rice blast /
- real-time PCR /
- gene expression and regulation