蛋白质电泳在杂交水稻种子纯度鉴定中的应用

基金项目:

福建省重点科技项目(2003N032)

Application of protein electrophoresis on the seed identification of hybrid rice

1.
College of Life Sciences, Fujian Agricultural and Forestry University, Fuzhou, Fujian 350002, China;
2.
Laiwu Vocational College Laiwu, Shandong, Laiwu, Shandong 271100, China

摘要: 以4个三系配套杂交水稻组合及7个杂交稻F1代种子为试验材料,提取其醇溶蛋白和盐溶蛋白,利用SDS-PAGE技术进行图谱分析。结果表明:醇溶蛋白的多态性较弱,差异不大,蛋白质特征带不明显,不适合用于杂交水稻组合和杂交种子的真实性和纯度的检测;而盐溶蛋白的多态性丰富,差异明显,蛋白质特征带稳定,杂交水稻种子蛋白质电泳鉴定与田间小区种植鉴定结果基本吻合。SDS-PAGE技术简便快捷、分离效果好,是一种经济有效的技术,可作为杂交水稻F1代种子纯度蛋白质指纹图谱鉴定。
- 杂交水稻 /
- 种子 /
- 醇溶蛋白 /
- 盐溶蛋白 /
- SDS-PAGE /
- 纯度鉴定
Abstract: Salt-and alcohol-soluble proteins extracted from the seeds of four different hybrid rice and their three lines,as well as seven other F1 hybrid progenies were analyzed by means of SDS-PAGE.The results showed that the polymorphism of alcohol-soluble proteins was not significant enough for authenticity and purity assessment.However,the salt-soluble proteins had a high polymorphism with stable and specific bands.The experiments demonstrated that SDS-PAGE was a simple,rapid,effective and inexpensive method for the identification of hybrid rice seeds.
- hybrid rice /
- seeds /
- alcohol-soluble protein /
- saltsoluble protein /
- SDS-PAGE /
- seed identification

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