摘要:
选用无猪瘟抗体原料兔作试验,研究酷暑对兔体温变化及注射猪瘟兔化弱毒脾淋乳浆液后产生定型热比率的影响。结果表明,当兔体周围气温达38℃时,100%的兔体温超过41℃;当环境温度达35.5℃时,100%的兔体温超过40℃。这些试验兔群表现出不同程度的中暑症状。发生中暑的试验兔群,在22℃的GMP生产动物房内饲养3d后,每只注射含4000个发热单位的猪瘟兔化弱毒脾淋乳浆液,5个试验组所产生的定型热比率分别为47%、65%、73%、54%、63%;而饲养于17～25℃舒适气温中的无猪瘟抗体兔群,转移到22℃的GMP生产动物房,注射相同病毒量后,产生定型热比率为98%、96%。上述结果表明在猪瘟兔化弱毒脾淋疫苗生产中,GMP生产动物房必须给兔子提供最舒适的室温,原料兔在生产后期和运输过程中也应避免中暑。但中暑过的试验兔,若能产生定型热,其脾淋毒价仍符合制造活疫苗的要求。
Abstract:
Rabbits without Classical Swine Fever (CSF) antibody were employed to produce Classical Swine Fever Vaccine (CSFV) to study the effect of hot summer weather on rabbit body temperature and the rate of regular type CSF in rabbits injected with CSFV thick liquid of spleens and lymph nodes.The results showed that all rabbit body temperature was over 41℃ and 40℃ when the surrounding temperature was up to 38℃ and 35.5℃,respectively.In varying degrees these rabbits showed heliosis symptom.After raised at 22℃ in a GMP animal house for 3 days,they were divided into 5 groups to be injected with 4000 fever units of the CSFV.The rates of regular type CSF in the 5 groups of the inoculated rabbits were 47%,65%,73%,54% and 63%.The rates for the control groups were 96% and 98%.The rabbits in the control groups had no CSF antibody and were raised in the surrounding temperature of 17-25℃.They were injected with the same dosage of the CSFV after being transported to the environment at 22℃.The above result revealed that an optimum surrounding temperature was necessary when the rabbits were to be used for CSFV production.In addition,the subject rabbits should avoid heliosis during the late production process and transportation.However,rabbits with heliosis could still be used to produce CSFV from their spleens and lymph nodes if they had contracted the regular type CSF.
点击查看大图
下载:
