Optimization of PLB induction conditions for Oncidium
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摘要: 以蜜糖文心兰不同生长期的新芽为试材,研究影响文心兰原球茎诱导的主要因素,结果表明:以半开展新芽的顶芽或侧芽为外植体,用0.1%升汞溶液灭菌9min,待外植体成活长成第一代植株时,再进行剥茎尖处理诱导原球茎形成,采用这种方法,外植体成活率明显提高,并在最短时间内诱导形成原球茎(PLB)。适宜的PLB诱导培养基为改良1号+6-BA 2.0mg·L-1+NAA 0.2mg·L-1。Abstract: The main factors of protocorm-like body (PLB) induction of Onc.Sweet Sugarr were studied by using new shoots in different growth periods of the plant.The results showed that the terminal and the lateral buds of the semi-developed new shoots were better explants than others.The suitable method for the explant surface sterilization was to apply 0.1% HgCl2 for 9 minutes.After the explant became a primary plantlet,it was time to peel the shoot-tips for PLB induction.The explant survival rate could thus be significantly improved,and the PLBs could be induced in the shortest time possible.The formula of the optimal culture medium for PLB induction was the Gailang1 medium +6-BA 2.0 mg·L-1+NAA 0.2 mg·L-1.
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Key words:
- Oncidium /
- explant /
- shoot-tip /
- protocorm-like body (PLB)
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