嗜热脂肪土芽孢杆菌CHB1发酵培养基的优化

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基金项目:

福建省发改委资助项目(闽计高技[2003]37)

Optimum medium composition for Geobacillus stearothermophilus CHB1 fermentation

1.
Soil and Fertilizer Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013, China;
2.
College of Biological Science and Technology, Fuzhou University, Fuzhou, Fujian 350002, China

摘要: 以菌体生长量和pH值作为培养基优化的指标,对嗜热脂肪土芽孢杆菌CHB1发酵培养基成分进行了筛选与优化。结果表明,不同碳氮源对发酵液菌数影响相差较大,最佳培养基配比为:牛肉膏、大豆蛋白胨、NaCl、K2HPO4和KH2PO4;最佳摇瓶发酵配方为:牛肉膏0.5%,大豆蛋白胨0.9%,NaCl 0.2%,K2HPO4:KH2PO4为0.1%:0.075%,该培养基中培养CHB1比在发酵基础培养基中培养菌量提高10倍以上。
- 嗜热脂肪土芽孢杆菌CHB1 /
- 发酵培养基 /
- 优化
Abstract: Cell biomass and pH of Geobacillus stearothermophilus CHB1 in culture medium were used as indices in the optimization study.The results indicated that different carbon and nitrogen sources significantly affected the microbial growth.By using orthogonal experimentation design,the optimal medium composition was obtained: 0.5% beef extract,0.9% soy peptone,0.2% NaCl,0.1% K2HPO4 and 0.075% KH2PO4.It was found that the biomass resulted from the newly formulated medium was ten times greater than that from the standard fermentation medium.
- Geobacillus stearothermophilus CHB1 /
- fermentation medium /
- optimization

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