Genetic Analysis and Gene Mapping of a Spotted Leaf Mutant W1764 in Rice
-
摘要: 从籼稻品种明恢86转基因后代中发现1个非T-DNA插入的叶斑突变体W1764。突变体在分蘖后期最先从基部叶叶尖处开始出现褐色斑点,随着植株的生长,斑点逐渐从叶尖扩展到整个叶片,同时从下到上叶片依次出现表型,至成熟时几乎遍布整个植株。遗传学分析表明,该突变体表型受单个隐性核基因控制。以突变体W1764和粳稻品种02428杂交构建F2群体,用其中突变表型的136株个体进行基因定位,将W1764的突变位点初步定位在第12染色体长臂的分子标记NSSR1229和L05-78之间1.9cM内。初步鉴定为1个新的叶斑突变基因,暂命名为spl30(t)。Abstract: A non-T-DNA insertion spotted leaf mutant W1764 was identified in a transgenic offspring of rice(Oryza sativa L.)indica cv.Minghui86.The spot phenotype was first appearing at the tip part of the lowest leaf at late stage of tillering.As the plant growing,the spot spread from tip part to the whole leaves from lower leaves to the upper ones.When the mutant plant matured,the spot spread to the whole plant.Genetic analysis showed that the mutant phenotype was controlled by a single recessive gene.F2 population build up with mutant W1764 and hybrid rice varieties 02428,and the 136 mutant phenotypes were used for gene targeting,we mapped the mutant gene in 1.9 cM interval between DNA marker NSSR1229 and L05-78 on the long arm of chromosome 12.We temporarily named the gene as spotted leaf 30(t)[spl30(t)].
-
Key words:
- Rice /
- Spotted leaf mutant /
- Genetic analysis /
- Gene mapping
-
[1] YAMANOUCHI U, YANO M, LIN H, et al.A rice spotted leaf gene, Spl7, encodes a heat stress transcription factor protein[J].Proc Natl Acad Sci,2002, 99(11):7530-7535. [2] MORI M, TOMITA C, SUGIMOTO K, et al.Isolation and molecular characterization of a Spotted leaf 18 mutant by modified activation-tagging in rice[J].Plant Mol Biol,2007, 63(6):847-860. [3] [4] QIAO Y, JIANG W, LEE J, et al.SPL28encodes a clathrin-associated adaptor protein complex 1, medium subunit 1(A P1M1)and is responsible for spotted leaf and early senescence in rice(Oryza sativa)[J].The New phytologist, 2010, 185(1):258-274. [5] MICHELMORE RW, PARAN I, KESSELI RV.Identification of markers linked to disease-resistance genes by bulked segregant analysis : A rapid method to detect markers in specific genomic regions by using segregating populations[J]. Proc Natl Acad Sci,1991, 88(21):9828-9832. [6] -
点击查看大图
计量
- 文章访问数: 3400
- HTML全文浏览量: 103
- PDF下载量: 944
- 被引次数: 0
下载:
