Optimizing of Extraction for Active Substances from Metabolites of Brevibacillus brevis FJAT-0809-GLX
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摘要: 为分析短短芽胞杆菌FJAT-0809-GLX代谢产物中的活性物质成分,对其提取方法进行了优化,大孔树脂提取法为最佳的提取方法,其活性物质得率为1.593gL-1,且抑菌活性高于氯仿萃取法所得的活性物质的活性。确定了短短芽胞杆菌FJAT-0809-GLX代谢产物活性物质收集的最佳方案为:将短短芽胞杆菌FJAT-0809-GLX发酵液常温3 600rmin-1离心30min,取上清液,按比例与40gL-1大孔树脂AmberliteXAD16混合,28℃、160rmin-1振荡4h后,填柱,并用水洗柱后,采用3倍洗脱体积的丙酮洗脱,收集丙酮洗脱液,40℃旋转蒸发进行浓缩,所得物质即为短短芽胞杆菌FJAT-0809-GLX代谢产物的活性物质。Abstract: Different extraction methods were compared in extracting of active substances from metabolites of Brevibacillus brevis FJAT-0809-GLX.It macroporous tesin method provided the best effect in extraction with 1.593 gL-1.Antibacterial activities of the extract was higher in it by macroporous resin method than it by the chloroform.The optimal procedures applied for extraction of active substances were: centrifuging the fermentation broth of FJAT-0809-GLX at 3600 rpm for 30 min,mixing the supernatant with 40 gL-1 macroporous resin(AmberliteXAD16)and then vortexing for 4h at 160 rpm,28℃;filling the column with the mixture and eluting it with acetone in the ratio of 3 times in given volum of extraction.The eluent was collected and concentrated rotary evaporation at 40℃.
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