The Effect on Proliferation Function of B Lymphocyte After Co-infection with MDRV and H9 AIV
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摘要: 以番鸭呼肠孤病毒(MDRV)或/和H9亚型水禽流感病毒(AIV)人工感染8日龄健康雏番鸭,应用MTT法测定感染后7、14和21d番鸭血液、脾脏和法氏囊中的B淋巴细胞增殖功能的变化动态,探讨MDRV或/和H9亚型AIV对B淋巴细胞增殖功能的影响。结果显示8日龄番鸭人工感染后,MDRV感染、AIV感染和共感染组番鸭血液、法氏囊和脾脏的B淋巴细胞刺激指数均明显低于健康对照组,而且共感染组番鸭B淋巴细胞刺激指数低于MDRV感染和AIV感染组,表明MDRV和H9 AIV病毒感染均能抑制番鸭外周血液及体液免疫器官(法氏囊、脾脏)中B淋巴细胞增殖功能,MDRV与H9 AIV共感染加重B细胞免疫抑制程度。
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关键词:
- 番鸭呼肠孤病毒 /
- H9亚型水禽流感病毒 /
- 共感染 /
- B淋巴细胞 /
- 增殖功能
Abstract: To reveal the immunosuppression mechanisms,eight-day-old Muscovy ducklings,were challenged with Muscovy duck reovirus(MDRV)and avian influenza virus(H9AIV)individually and simultaneously.The changes of proliferation of B lymphocytes in the blood,spleens and bursa were determined by MTT method 7 days,14 days and 21 days after the Muscovy ducklings were challenged.The results show that B lymphocyte stimulation indexes in the blood,bursa and spleen of the challenged group were significantly lower than that in control group,and B lymphocytes stimulation index in co-infection group was the lowest.So B lymphocytes proliferation of peripheral blood and humoral immune organs(bursas,spleens)in Muscovy ducklings was inhibited by both MDRV and H9AIV,and co-infection with MDRV and H9AIV could aggravate this immunosuppression.-
Key words:
- MDRV /
- H9AIV /
- Co-infection /
- B lymphocytes /
- Proliferation
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