基于rDNA ITS分析的假高粱鉴定方法

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基金项目:

国家质量检验检疫监督总局科技项目(IK2002059)

Application of ribosoral DNA internal transcribed spacer to identification of Sorghum halepensea

1.
Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou, Fujian 350001, China;
2.
Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China

摘要: 通过对供试的假高粱和同属近似种拟高粱、明福1号的rDNA ITS区进行PCR扩增和RFLP分析,结果表明,明福1号、拟高粱和假高粱三者均有850 bp左右条带,而且假高粱在650 bp和200 bp附近均有两条谱带, 而明福1号、拟高粱在650 bp和200 bp附近只有一条清楚谱带,与测序比对结果一致。
- 假高粱 /
- ITS区 /
- RFLP
Abstract: The PCR amplified ITS fragments were analyzed by restriction endonuclease digestion (RFLP).Results showed that the RFLP patterns of S.halepensem (L.) Pers which yielded two bands around 650 bp and two bands around 200 bp.But significantly different from the RFLP patterns of S.sp.and S.propinguum (Kunth.) Hitche which yielded one band around 650 bp and one band around 200 bp.The results were consistent with the fact which S.halepensem had two selective identify sites of PmaC I while S.sp.and S.propinguum each had one.Therefore, it could differentiate S.propinguum, S.sp.and S.halepensem (L.) Pers by PmaC I.
- Sorghum halepensea (L.) pers /
- internal transcribed spacer /
- restriction fragment length polymorphism

[1]	SUN Y,SKINNER D Z,LIANG G H,et al.Phylogenetic analysis of Sorghum and related taxa using internal transcribed spacers of nuclear ribosomal DNA[J].Theor Appl Genet,1994.89:26-32.

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