Isolation and identification of E.coli pathogens from 9 swine farms in Fujian Province
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摘要: 从福建省的福州、福清、漳州、莆田、邵武、厦门、泉州等9个发病猪场采集病料,共分离出14株病原菌,经福建省防疫站菌种鉴定和用大肠杆菌K88、K99的特异引物进行PCR扩增,结果表明所分离的大肠杆菌中 10株为普通大肠埃希氏菌,2株为β溶血性菌株。2株为大肠杆菌K88。纯化菌株的药敏试验结果表明,和大肠杆菌标准株K88、K99相比,不同猪场分离的大肠杆菌对抗菌素的耐药性都有较大增强。Abstract: Forteen strains of E.coli pathogens were isolated from 9 swine farms in Fuzhou, Fuqing, Zhangzhou, Putian, Shaowu, Xiamen, Quanzhou of Fujian, and were identified by Fujian Epidemic Prevention Station and analyzed by PCR with E.coli K88 and K99 specific PCR primers.The result showed that 10 strains belonged to normal E.coli.2 strains belonged to β-hemolytic E.coli and 2 strains belonged to E.coli K88.Insecticide tolerance of the isolated strains were higher than that of standard strains E.coli K88, K99 by drugsensitive test.
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Key words:
- Fujian Province /
- swine farm /
- E.coli /
- isolation and identification
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[1] 王开功,周碧君,冯莹,等.贵州仔猪大肠杆菌分离物K88菌毛的鉴定[J].贵州农学院学报,1997,16(2):20-23. [2] 许崇波,卫广森,王成文,等.表达大肠杆菌K88ac-ST1-LTB融合蛋白工程菌株的免疫原性研究[J].中国预防兽医学报,2003,25(1):9-11. -
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