Isolation and identification of the bacteria causing acute epidemic septicemia in cultured fresh water fishes and the treatment
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摘要: 2003年下半年福建省龙海市许多养鱼场发生一起以出血为主的家养鱼暴发性传染病,对其进行了病原分离鉴定。通过常规细菌学鉴定和应用 ATB 全自动鉴定系统,从濒死的鲢鱼和罗非鱼的实质性脏器中各分离出1株迟缓爱德华氏菌(Edwardstella tarda),同时还从鲢鱼中分离出1株弗氏枸掾酸杆菌(Citrobacter freandn)。用这些菌人工感染小鼠和健康鱼,表明这些菌对小鼠有极强的毒力,对鱼表现出与自然发病鱼类似的病症,并回收到与接种菌一致的细菌。选择敏感性高的丙沙星、氟哌酸、菌必治交叉使用．同时搞好周围环境卫生,有效地控制了病情。Abstract: Systemic investigations on the pathogen of epidemic septicemia,a new infectious disease in fishes(mainly silver carps,tilapia)which occurred among cultured fishes in South China in summer to early-autumn of 2003 were carried out.Two strains of Edwardstella tarda(Et)and a strain of Citrobacter freundn(Cf)were isolated from kidney of moribund fishes with hemorrhage in dermis,vent,fin,gill and internal organs.Four-tube method and clinic symptom indicated that two isolates belong to Et and a isolate Cf.In addition.VITEK-JR30 system was used to identify these isolates and reconfirmed the results.Pathogenic test documented that the bacteria are the causative agents of the disease.After experimentally infected by the bacteria via intraperitoneal inoculation,fishes died of hemorrhagic scpticemia,similar to naturally infected fish.The piscine diseases were effectively controlled by using sensitive drugs which had been proven to be strongly susceptive to these strains.
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Key words:
- Fish /
- Edwardsiella tarda /
- Citrobacter freundii /
- Isolation and Identification /
- Treatment
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[1] 陈昌福,毛芝娟,王敏.福建爱德华氏菌对土霉素、氯霉素和链霉素的感受性、耐药性研究[J].淡水渔业,1996,26(3):3-5. [2] 熊清明,陆承平.迟缓爱德华菌(Edwardsiella tarda)对小鼠和剑尾鱼的免疫保护试验[J].中国兽医学报,2002,22(3):251-252. -
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