Several factors for culture of isolating microspores of Chinese cabbage
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摘要: 以早熟和中晚熟大白菜为试材,在福州环境条件下,研究基因型和供体植株的生理状况对小孢子胚胎发生和植株再生的影响。试验结果表明,两种类型的大白菜小孢子胚胎诱导率无明显差异,不同品种材料的小孢子胚胎发生频率存在很大差异;供体植株采蕾期的气温条件是小孢子胚胎发生的关键因素,采蕾之前3d的气温在14℃/24℃(夜/昼)最适宜小孢子发育;盛花期的花蕾和供体植株营养状况良好,有利于胚胎发生及植株再生。Abstract: The varieties of early-mature and middle-late-mature Chinese cabbage were used to study the influence of genotype and physioloyical status of donor plants on microspore embryogenesis and plant regeneration.The results showed that inducement ratio of the embryoid was significantly different among the different donor plants and the embryo productivity had no significant difference between the earty-mature varieties and middle-late-maturing ones.The temperature from three days before bud plucking to the plucking day was a key factor and 14℃/24℃ (night/day)was most suitable to induce microspore embryogenesis.The bud in full florescence and the donor plant with good nutritional status were also beneficial to induce microspore embryogenesis and plant regeneration.
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Key words:
- Chinese cabbage /
- Microspore culture /
- Genotype /
- Physioloyical status
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[1] 曹鸣庆,刘凡.芸苔属蔬菜游离小孢子培养研究进展[C].园艺学年评,1996,2:63-90. [2] 曹鸣庆,李岩,刘凡.基因型和供体植株生长环境对大白菜游离小孢子胚胎发生的影响[J].华北农学报,1993,8(4):1-6. -
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