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一例半番鸭感染鸭瘟的病原分离鉴定及病理组织学观察

张瑞 刘荣昌 陈长福 黄瑜 程龙飞 傅光华 施少华 陈红梅 万春和 傅秋玲

张瑞,刘荣昌,陈长福,等. 一例半番鸭感染鸭瘟的病原分离鉴定及病理组织学观察 [J]. 福建农业学报,2021,36(3):312−318 doi: 10.19303/j.issn.1008-0384.2021.03.009
引用本文: 张瑞,刘荣昌,陈长福,等. 一例半番鸭感染鸭瘟的病原分离鉴定及病理组织学观察 [J]. 福建农业学报,2021,36(3):312−318 doi: 10.19303/j.issn.1008-0384.2021.03.009
ZHANG R, LIU R C, CHEN C F, et al. Pathogen Identification and Tissue Histopathology on Plague Virus-infected Mule Ducks [J]. Fujian Journal of Agricultural Sciences,2021,36(3):312−318 doi: 10.19303/j.issn.1008-0384.2021.03.009
Citation: ZHANG R, LIU R C, CHEN C F, et al. Pathogen Identification and Tissue Histopathology on Plague Virus-infected Mule Ducks [J]. Fujian Journal of Agricultural Sciences,2021,36(3):312−318 doi: 10.19303/j.issn.1008-0384.2021.03.009

一例半番鸭感染鸭瘟的病原分离鉴定及病理组织学观察

doi: 10.19303/j.issn.1008-0384.2021.03.009
基金项目: 国家水禽产业技术体系项目(CARS-42);福建省科技计划公益类专项(2020R1026001、2020R10260013);福建省农业科学院特色现代农业产业发展自由探索科研项目(AA2018-12)
详细信息
    作者简介:

    张瑞(1989−),男,硕士研究生,研究方向:预防兽医学(E-mail:759893483@qq.com

    刘荣昌(1988−),男,助理研究员,硕士,研究方向:动物病原学与病理学(E-mail:liurongc@foxmail.com

    通讯作者:

    黄瑜(1965−),男,研究员,博士,研究方向:畜禽传染病(E-mail:huangyu_815@163.com

  • 中图分类号: S 834.89

Pathogen Identification and Tissue Histopathology on Plague Virus-infected Mule Ducks

  • 摘要:   目的  2020年8月,福建省福州地区某鸭场饲养的半番群发病,发病率约为65%,病死率高达90%以上,感染鸭皮肤表面可见大量出血,为了明确其病原和病理组织学特征。  方法  分别采集发病鸭的肝脏、脾脏、胰腺、肾脏、皮肤等组织进行鸭常见病原检测、病毒分离鉴定、基因序列测定分析及病理组织学观察。  结果  病原检测结果显示,所采集组织仅鸭瘟病毒PCR检测呈阳性,其余病原均为阴性;对阳性样品进行病毒分离鉴定,结果从发病鸭内脏组织和皮肤中分离出鸭瘟病毒,命名为FJ2020176。对新分离鸭瘟病毒FJ2020176株UL2基因进行序列测定与分析发现,其与鸭瘟强毒株CHv株、CV株和2085株的核苷酸同源性为97.8%~99%;与疫苗株VAC株、Attenuatedstrain 1株和Attenuated strain 2株等弱毒株相比,FJ2020176株在UL2基因中存在528 bp的核苷酸插入,表明该毒株为鸭瘟病毒强毒株。病理组织学观察显示,感染鸭可见肝脏局灶性坏死、出血;脾脏组织白髓减少,淋巴细胞坏死、脱落;肾脏出血、淤血;法氏囊出血,淋巴小结坏死;皮下出血。  结论  从皮肤大量出血的发病鸭中分离到1株鸭瘟病毒强毒株,表明皮肤出血亦为鸭瘟病例的临床特征,以上结果为临床上鸭瘟的诊断提供了新的试验数据。
  • 图  1  发病鸭鸭剖检病变图

    注:A:头部;B:皮肤;C:肝脏。

    Figure  1.  Necropsy lesions in diseased ducks.

    Note: A: head; B: skin; C: liver.

    图  2  临床采集样品的PCR检测

    注:M:DNA 2 000 Marker;1:阳性对照;2:阴性对照;3:鸭瘟病毒(肝脏);4:鸭瘟病毒(脾脏);5:鸭瘟病毒(心脏);6:鸭瘟病毒(肾脏);7:鸭瘟病毒(法氏囊);8:鸭瘟病毒(皮肤);9.禽流感病毒;10:新城疫病毒;11:鸭肝炎病毒;12:禽坦布苏病毒;13:水禽细小病毒;14:鸭3型腺病毒;15:禽4型腺病毒;16:呼肠孤病毒;17:鸭星状病毒;18:鸭圆环病毒。

    Figure  2.  PCR detection on clinical samples

    Note: M: DNA 2000 marker; 1: positive control; 2: negative control; 3: duck plague virus on liver; 4: duck plague virus on spleen; 5: duck plague virus on heart; 6: duck plague virus on kidney; 7: duck plague virus on bursa of Fabricius; 8: duck plague virus on skin; 9: avian influenza virus; 10: Newcastle disease virus; 11: duck hepatitis virus; 12: avian Tembusu virus; 13: waterfowl parvovirus; 14: duck adenovirus 3; 15: avian adenovirus 4; 16: reovirus; 17: duck astrovirus; 18: duck circovirus.

    图  3  临床采集样品的PCR检测

    注:M:DNA 2 000 Marker;1:阳性对照;2:阴性对照;3:内脏样品鸭胚传代鸭瘟;4:皮肤样品鸭胚传代鸭瘟;5:禽流感病毒;6:新城疫病毒;7:鸭肝炎病毒;8:禽坦布苏病毒;9:水禽细小病毒;10:鸭3型腺病毒;11:禽4型腺病毒;12:呼肠孤病毒;13:鸭星状病毒;14:鸭圆环病毒。

    Figure  3.  PCR detection on clinical samples

    Note: M: DNA 2000 marker; 1: positive control; 2: negative control; 3: duck plague passage from duck embryo in viscera samples; 4: duck plague passage from duck embryo in skin samples; 5: avian influenza virus; 6: Newcastle disease virus; 7: duck hepatitis virus; 8: avian Tambusu virus; 9: waterfowl parvovirus; 10: duck adenovirus 3; 11: avian adenovirus 4; 12: reovirus; 13: duck astrovirus; 14: duck circovirus.

    图  4  分离病毒的PCR扩增结果

    注:M:DNA 2 000 Marker;1:阳性对照;2:阴性对照;3:FJ2020176。

    Figure  4.  PCR amplification result on isolated virus

    Note: M: DNA 2000 marker; 1: positive control; 2: negative control; 3: FJ2020176.

    图  5  鸭瘟病毒UL2基因同源性分析

    Figure  5.  Homology on UL2 gene of duck plague virus

    图  6  感染鸭组织病理切片

    注:A. 肝脏(×100);B. 肝脏(×400);C. 脾脏(×100);D. 脾脏(×400);E. 肾脏(×100);F. 肾脏(×400)G. 法氏囊(×100);H. 法氏囊(×400);I. 皮肤(×100);J. 皮肤(×400)。

    Figure  6.  Pathological slices of infected duck tissues

    Note: A: liver (×100). B: liver (×400). C: spleen (×100). D: spleen (×400). E: kidney (×100). F: kidney (×400). G: bursa of fabricius (×100). H: bursa of fabricius (×400). I: skin (×100). J: skin (×400).

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  • 收稿日期:  2020-11-19
  • 修回日期:  2020-12-31
  • 网络出版日期:  2021-02-08
  • 刊出日期:  2021-03-31

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