外源褪黑素对低温逆境下三角梅生理特性和细胞壁组分的影响

胡牮; 林雅楠; 吴楠; 蒋诗睿; 宋杨; 张建新; 郑玲霞; 陈祺月; 刘鹏

外源褪黑素对低温逆境下三角梅生理特性和细胞壁组分的影响

胡牮^1,,
林雅楠²,
吴楠²,
蒋诗睿²,
宋杨²,
张建新¹,
郑玲霞²,
陈祺月²,
刘鹏^2, ,

1.
丽水职业技术学院　浙江丽水　323000
2.
浙江师范大学　植物学实验室　浙江金华　321004

基金项目: 国家自然科学基金项目(32001224，30540056); 浙江省公益研究计划项目(LGN19C140009);浙江大花园建设研究院2021年专项课题(DHYB1001)

详细信息

作者简介:
胡牮（1985 —），男，硕士，副教授，主要从事植物逆境生理、园林植物及其应用研究，E-mail：hudainiu@163.com

通讯作者:
刘鹏(1965−)，男，博士，教授，主要从事植物逆境生理、环境污染与保护及植物生理生态研究，E-mail:sky79@zjnu.cn

中图分类号: S685.99
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出版历程
- 收稿日期: 2024-01-04
- 修回日期: 2024-03-07
- 网络出版日期: 2024-05-08

Effects of Exogenous Melatonin on Physiological Characteristics and Cell Wall Components of Bougainvillea Under Cold Stress

1.
Lishui Vocational and Technical College, Lishui, Zhejiang　323000, China
2.
Key Laboratory of Botany, Zhejiang Normal University, Jinhua, Zhejiang　321004, China

摘要

摘要: 目的研究褪黑素对低温逆境下三角梅生理生化及细胞壁组分上的调控作用，为缓解低温对三角梅生长造成的不利影响提供理论依据。方法以耐冷性不同的三角梅安格斯、口红、变色龙3个品种为供试材料，在4 ℃低温下对三角梅植株叶面施加不同浓度（0、50、100、150 µmol·L^–1）的外源褪黑素，测定各处理组生长周期（0、7、14、21 d）过程中三角梅叶面积、叶绿素含量、叶绿素荧光参数[PSⅡ最大光化学量子产量（F_v/F_m）、表观电子传递速率（ETR）、光化学淬灭系数（qP）、非光化学淬灭系数（qN）]、抗氧化酶[超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）]活性、膜脂过氧化产物[超氧阴离子（O₂⁻）产生速率、过氧化氢（H₂O₂）含量]、渗透调节物质[可溶性糖（SS）、可溶性蛋白（SP）]含量、细胞壁组分[螯合型果胶（CSP）、碱溶性果胶（SSP）、半纤维素（HC）和纤维素（CE）]含量。结果低温逆境下三角梅的正常生长发育受阻，叶绿素荧光参数失衡，膜脂过氧化加剧，细胞壁组分受到影响。而施加100 µmol·L^–1的褪黑素能够有效地提升三角梅的生理活性，改善植株萎蔫程度，F_v/F_m与ETR降幅减小；抗氧化酶活性显著增加，安格斯较于0 d的SOD、POD、CAT最大可增加96.45%、104.35%、73.11%；H₂O₂含量、O₂⁻生成速率对比同周期MT₀最多下降21.07%、26.85%；细胞壁组分中CSP、SSP上升幅度增加，较于0 d最多可上涨22.55%、43.08%。结论明确了外源褪黑素在生理特性方面对不同品种三角梅冷害的调控作用，外源施加适宜浓度的褪黑素可以有效缓解低温对三角梅生长的抑制，减轻光系统损伤，增强抗氧化酶活性，减缓膜脂氧化，并调节细胞壁各组分含量以维持其结构整体稳定，从而降低低温伤害，为探索褪黑素对冷害下植物的调控作用提供新思路。
- 褪黑素 /
- 三角梅 /
- 低温逆境 /
- 生理响应 /
- 细胞壁组分
Abstract: : Objective To study the regulation effect of melatonin on physiological and biochemical characteristics and cell wall components of Bougainvillea under low temperature stress, and to provide a theoretical basis for alleviating the adverse effects of low temperature on the growth of Bougainvillea. Method The leaves of Bougainvillea were treated with exogenous melatonin at different concentrations (0, 50, 100, 150 µmol·L^–1) under low temperature of 4°C. The leaf area, chlorophyll content, chlorophyll fluorescence parameters [optimal/maximal quantum yield of PSⅡ (F_v/F_m), apparent electron transfer rate (ETR), photochemical quenching coefficient (qP), non-photochemical quenching coefficient (qN)], antioxidant enzyme activities [superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT)], membrane lipid peroxidation products [production rate of superoxide anion (O₂⁻), hydrogen peroxide (H₂O₂) content], osmotic adjustment substances [soluble sugar (SS), soluble protein (SP)], and cell wall components [chelate pectin (CSP), alkali-soluble pectin (SSP), hemicellulose (HC) and cellulose (CE)] of Bougainvillea were measured during the growth cycle of each treatment group (0, 7, 14, 21 d). Result Under low temperature stress, the normal growth and development of Bougainvillea were hindered, chlorophyll fluorescence parameters were unbalanced, membrane lipid peroxidation was intensified, and cell wall components were affected. The application of 100 µmol·L⁻¹ melatonin was the most effective way to enhance the physiological activity, improve the degree of wilting, and decrease the F_v/F_m and ETR. The activity of antioxidant enzymes increased significantly, and SOD, POD and CAT increased 96.45%, 104.35% and 73.11% in Angus compared with 0 d. H₂O₂ content and O₂⁻ production rate decreased by 21.07% and 26.85% compared with MT₀ in the same period. The increase of CSP and SSP in cell wall components increased by 22.55% and 43.08% compared with 0 d. Conclusion In conclusion, This study clarified the regulatory effects of exogenous melatonin on the physiological characteristics of different varieties of Bougainvillea cold damage. Exogenous application of melatonin can effectively alleviate the inhibition of low temperature on the growth of Bougainvillea, reduce photosystem damage, enhance antioxidant enzyme activity, slow down membrane lipid oxidation, and regulate the content of cell wall components to maintain overall structural stability, thereby reducing low temperature damage. This provides new ideas for exploring the regulatory role of melatonin on plants under cold damage.
- melatonin /
- Bougainvillea /
- low temperature stress /
- physiological response /
- cell wall components

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图 1 MT处理对低温下三角梅表观性状的影响

Figure 1. Effects of MT treatment on apparent character of Bougainvillea at low temperature

EA:Bougainvillea Elizabeth Angus；SC：Bougainvillea Snow Cap；MH：Bougainvillea Marry Helen，as below.

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图 2 MT处理对低温下三角梅叶绿素含量的影响

不同小写字母表示同一品种不同处理间差异显著（P＜0.05），下同。

Figure 2. Effects of MT treatment on chlorophyll content of Bougainvillea at low temperature

Data with different lowercase indicate significant differences between treatments on same Bougainvillea variety at P＜0.05，as below.、

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图 3 MT处理对低温下三角梅叶绿素荧光参数的影响

Figure 3. Effect of MT treatment on on chlorophyll fluorescence parameters of Bougainvillea under low temperature

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图 4 MT处理对低温下三角梅过氧化氢含量和超氧阴离子产生速率的影响

Figure 4. Effect of MT treatment on hydrogen peroxide content and superoxide anion production rate of Bougainvillea under low temperature

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图 5 MT处理对低温下三角梅抗氧化酶系统的影响

Figure 5. Effect of MT treatment on antioxidant oxidase system of Bougainvillea under low temperature

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图 6 MT处理对低温下三角梅可溶性糖和可溶性蛋白含量的影响

Figure 6. Effect of MT treatment on contents of soluble sugar and soluble protein in Bougainvillea under low temperature

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图 7 MT处理对低温下三角梅细胞壁组分的影响

Figure 7. Effect of MT treatment on cell wall components of Bougainvillea under low temperature

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