• 中文核心期刊
  • CSCD来源期刊
  • 中国科技核心期刊
  • CA、CABI、ZR收录期刊

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

簇生稻穗发育及细胞周期相关基因的表达分析

陈丽萍 蒋家焕 郑燕梅 朱永生 王颖姮 林强 谢鸿光 罗曦 蔡秋华 谢华安 张建福

陈丽萍,蒋家焕,郑燕梅,等. 簇生稻穗发育及细胞周期相关基因的表达分析 [J]. 福建农业学报,2021,36(3):249−254 doi: 10.19303/j.issn.1008-0384.2021.03.001
引用本文: 陈丽萍,蒋家焕,郑燕梅,等. 簇生稻穗发育及细胞周期相关基因的表达分析 [J]. 福建农业学报,2021,36(3):249−254 doi: 10.19303/j.issn.1008-0384.2021.03.001
CHEN L P, JIANG J H, ZHENG Y M, et al. Expressions of Panicle Development- and Cell Cycle-related Genes in Rice with Clustered Spikelets [J]. Fujian Journal of Agricultural Sciences,2021,36(3):249−254 doi: 10.19303/j.issn.1008-0384.2021.03.001
Citation: CHEN L P, JIANG J H, ZHENG Y M, et al. Expressions of Panicle Development- and Cell Cycle-related Genes in Rice with Clustered Spikelets [J]. Fujian Journal of Agricultural Sciences,2021,36(3):249−254 doi: 10.19303/j.issn.1008-0384.2021.03.001

簇生稻穗发育及细胞周期相关基因的表达分析

doi: 10.19303/j.issn.1008-0384.2021.03.001
基金项目: 福建省自然科学基金项目(2018J05052);福建省农业科学院青年科技英才项目(Y2017-1)
详细信息
    作者简介:

    陈丽萍(1986−),女,博士,助理研究员,主要从事水稻分子育种研究(E-mail:373200630@qq.com

    通讯作者:

    谢华安(1941−),男,研究员,主要从事杂交水稻育种研究(E-mail:huaanxie@163.com

    张建福(1971−),男,博士,研究员,主要从事水稻分子育种研究(E-mail:jianfzhang@163.com

  • 中图分类号: S 511

Expressions of Panicle Development- and Cell Cycle-related Genes in Rice with Clustered Spikelets

  • 摘要:   目的  穗粒数的增加可以直接提高水稻的产量,簇生穗能增加穗粒数和着穗密度。为考察簇生稻品种内江P164与对照单粒稻品种9311中OsCl-6等穗发育及细胞周期相关基因的表达是否存在显著差异,探索通过调控品种中特定基因的表达量来指导水稻生产,为高产水稻品种选育提供理论依据。  方法  分别取种植于网室大田的簇生稻品种内江P164和对照单粒稻品种9311的幼穗进行RNA提取、qRT-PCR分析及相关数据分析。  结果  qRT-PCR结果显示,对于所选取的几个幼穗发育时期,OsCl-6的表达量在内江P164的幼穗中的表达量均高于9311。细胞周期依赖激酶类基因在9311中的表达高于内江P164,而B类细胞周期蛋白则在内江P164中的表达高于9311。Lax1、OsWOX3、RFL等穗发育相关基因在内江P164中的表达低于9311,而MFS1和CL-4的在内江P164中的表达则高于9311。  结论  簇生穗基因OsCl-6等穗发育相关基因及B类细胞周期蛋白在簇生稻品种内江P164与对照单粒稻品种9311中的表达存在显著差异,OsCl-6或通过表达量上调来抑制二次枝梗的发育等,将为深入挖掘穗发育相关基因之间的关系及簇生穗基因OsCl-6的分子机理研究提供参考。
  • 图  1  幼穗发育不同时期OsCl-6的表达量分析

    Figure  1.  Expression of OsCl-6 in young panicles at developmental stages

    图  2  幼穗中细胞周期相关基因的表达分析

    Figure  2.  Expressions of cell cycle-related genes in young panicle

    图  3  幼穗枝梗发育相关基因的表达分析

    Figure  3.  Expressions of panicle branch development-related genes in young panicle

    表  1  9311和内江P164的农艺性状比较

    Table  1.   Agronomic characteristics of 9311 and Neijiang P164

    品种
    Variety
    株高
    Plant
    height/cm
    分蘖数
    Tiller
    number
    一次枝梗数
    primary
    branch number
    二次枝梗数
    Secondary
    branche number
    穗粒数
    Spikelet number
    per panicle
    穗长
    Panicle
    length/cm
    着粒密度
    seed
    density/10 cm
    花粉可育率
    Fertility rate
    of pollen/%
    9311 98.57 10.3 15.1 35.4 191.5 21.16 90.9 97.1
    内江 P164
    Neijiang P164
    125.24** 9.6 12.7* 21.0** 154.8** 23.42* 65.5** 96.4
    注:*和**分别表示在0.05和0.01水平上差异显著。下同。
    Note: * and **represent significant at 0.05 and 0.01 probability level, respectively. Same for the following.
    下载: 导出CSV
  • [1] 张现伟, 李经勇, 郑家奎, 等. 复粒稻种质资源及其遗传育种利用 [J]. 植物学报, 2013, 48(4):438−446.

    ZHANG X W, LI J Y, ZHENG J K, et al. Germplasm resources and genetic breeding utilization of multiple-grain rice [J]. Chinese Bulletin of Botany, 2013, 48(4): 438−446.(in Chinese)
    [2] 邱箭, 李勤, 李国林, 等. 复粒稻恢复系选育及利用初报 [J]. 中国种业, 2014(12):70−71.

    QIU J, LI Q, LI G L, et al. Breeding and utilization of restorer lines in multigrain rice [J]. China Seed Industry, 2014(12): 70−71.(in Chinese)
    [3] 周鹏, 邓其明, 涂诗航, 等. 水稻穗部簇生突变体(Cl-dz)的遗传分析与初步定位 [J]. 福建农业学报, 2012, 27(5):481−484. doi: 10.3969/j.issn.1008-0384.2012.05.006

    ZHOU P, DENG Q M, TU S H, et al. Genetic analysis and gene mapping of the clustered spikelets mutant (Cl-dz) of rice [J]. Fujian Journal of Agricultural Sciences, 2012, 27(5): 481−484.(in Chinese) doi: 10.3969/j.issn.1008-0384.2012.05.006
    [4] 苏德文, 郑燕梅, 何炜, 等. 水稻簇生穗突变体基因研究进展 [J]. 福建农业学报, 2013, 28(9):931−937. doi: 10.3969/j.issn.1008-0384.2013.09.020

    SU D W, ZHENG Y M, HE W, et al. Progress on the gene of rice clustered spikelets (Cl) mutant [J]. Fujian Journal of Agricultural Sciences, 2013, 28(9): 931−937.(in Chinese) doi: 10.3969/j.issn.1008-0384.2013.09.020
    [5] 陈光毅, 张红宇, 徐培洲, 等. 一个水稻簇生穗突变体的遗传分析与基因定位 [J]. 中国农学通报, 2014, 30(27):258−264. doi: 10.11924/j.issn.1000-6850.2014-0646

    CHEN G Y, ZHANG H Y, XU P Z, et al. Genetic analysis and gene mapping of a clustered spikelets mutant in rice [J]. Chinese Agricultural Science Bulletin, 2014, 30(27): 258−264.(in Chinese) doi: 10.11924/j.issn.1000-6850.2014-0646
    [6] 姜玲, 姜宁, 曹黎明, 等. 水稻簇生穗基因CPB4的精细定位 [J]. 复旦学报(自然科学版), 2018, 57(6):757−766.

    JIANG L, JIANG N, CAO L M, et al. Fine-mapping CPB4 gene associated with clustered spikelets in rice [J]. Journal of Fudan University (Natural Science), 2018, 57(6): 757−766.(in Chinese)
    [7] ZHENG L Y, ZHU X D, QIAN Q, et al. Morphology and mapping analysis of rice (Oryza sativa L.) clustered spikelets (Cl) mutant [J]. Chinese Science Bulletin, 2003, 48(6): 559−562. doi: 10.1360/03tb9119
    [8] 龙小林, 向珣朝, 张艳丽, 等. 水稻簇生穗控制基因ts的定位及育种利用研究 [J]. 农业生物技术学报, 2014, 22(9):1123−1130. doi: 10.3969/j.issn.1674-7968.2014.09.009

    LONG X L, XIANG X C, ZHANG Y L, et al. Gene mapping and breeding utilization of rice (Oryza sativa L.) clustered spikelets control gene ts [J]. Journal of Agricultural Biotechnology, 2014, 22(9): 1123−1130.(in Chinese) doi: 10.3969/j.issn.1674-7968.2014.09.009
    [9] JIANG G H, XIANG Y H, ZHAO J Y, et al. Regulation of inflorescence branch development in rice through a novel pathway involving the pentatricopeptide repeat protein sped1-D [J]. Genetics, 2014, 197(4): 1395−1407. doi: 10.1534/genetics.114.163931
    [10] GUO M, YANG Y H, LIU M, et al. Clustered spikelets 4, encoding a putative cytochrome P450 protein CYP724B1, is essential for rice panicle development [J]. Chinese Science Bulletin, 2014, 59(31): 4050−4059. doi: 10.1007/s11434-014-0568-z
    [11] SONG S, WANG G F, HU Y, et al. OsMFT1 increases spikelets per panicle and delays heading date in rice by suppressing Ehd1, FZP and SEPALLATA-like genes [J]. Journal of Experimental Botany, 2018, 69(18): 4283−4293. doi: 10.1093/jxb/ery232
    [12] ZHANG Z Y, LI J J, YAO G X, et al. Fine mapping and cloning of the grain number per-panicle gene (Gnp4) on chromosome 4 in rice (Oryza sativa L.) [J]. Journal of Integrative Agriculture, 2011, 10(12): 1825−1833.
    [13] QI P, LIN Y S, SONG X J, et al. The novel quantitative trait locus GL3.1 controls rice grain size and yield by regulating Cyclin-T1; 3 [J]. Cell Research, 2012, 22(12): 1666−1680. doi: 10.1038/cr.2012.151
    [14] 宋建. 水稻细胞周期关键调节基因的全基因组分析及Orysa;DEL1功能的初步鉴定[D]. 泰安: 山东农业大学, 2008.

    SONG J. Genome-wide analysis of core cell cycle genes and function identification of Orysa; DEL1 in rice[D]. Tai’an: Shandong Agricultural University, 2008.
    [15] REN D Y, LI Y F, ZHAO F M, et al. MULTI-FLORET SPIKELET1, which encodes an AP2/ERF protein, determines spikelet meristem fate and sterile Lemma identity in rice [J]. Plant Physiology, 2013, 162(2): 872−884. doi: 10.1104/pp.113.216044
    [16] BARROCO R M, PERES A, DROUAL A M, et al. The syclin-dependent kinase inhibitor Orysa; KRP1 plays an important role in seed development of rice [J]. Plant Physiology, 2006, 142(3): 1053−1064. doi: 10.1104/pp.106.087056
    [17] WU Z G, JI J H, TANG D, et al. OsSDS is essential for DSB formation in rice meiosis [J]. Frontiers in Plant Science, 2015(6): 21.
    [18] 郑燕梅, 苏德文, 蒋家焕, 等. 水稻簇生穗基因OsCl-6的精细定位与候选基因分析 [J]. 农业生物技术学报, 2018, 26(7):1116−1123.

    ZHENG Y M, SU D W, JIANG J H, et al. Fine mapping and candidate gene analysis of clustered spikelet gene OsCl-6 in rice (Oryza sativa) [J]. Journal of Agricultural Biotechnology, 2018, 26(7): 1116−1123.(in Chinese)
    [19] LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2 [-Delta Delta C(T)] Method [J]. Methods (San Diego, Calif), 2001, 25(4): 402−408. doi: 10.1006/meth.2001.1262
    [20] IKEDA K, SUNOHARA H, NAGATO Y. Developmental course of inflorescence and spikelet in Rice [J]. Breeding Science, 2004, 54: 147−156. doi: 10.1270/jsbbs.54.147
    [21] 李经勇, 唐永群. 稗草DNA导入渝恢1351后代性状变异研究 [J]. 中国稻米, 2012, 18(2):27−29. doi: 10.3969/j.issn.1006-8082.2012.02.006

    LI J Y, TANG Y Q. Study on character variation of Panicum crus-galli DNA introduced Yuhui 1351 progeny [J]. China Rice, 2012, 18(2): 27−29.(in Chinese) doi: 10.3969/j.issn.1006-8082.2012.02.006
    [22] 俞法明, 杨曙东, 王晓峰, 等. 顶小穗簇生型早籼稻新种质的创制与鉴定 [J]. 核农学报, 2019, 33(5):848−854. doi: 10.11869/j.issn.100-8551.2019.05.0848

    YU F M, YANG S D, WANG X F, et al. Creation and identification of new germplasm of early indica rice with top spikelet cluster [J]. Journal of Nuclear Agricultural Sciences, 2019, 33(5): 848−854.(in Chinese) doi: 10.11869/j.issn.100-8551.2019.05.0848
    [23] FOUAD A S, ALSOBEAI S M. In silico characterization of a cyclin dependent kinase-A (CDKA) and its coding gene in some Oryza species [J]. Baghdad Science Journal, 2020, 17(3): 0760. doi: 10.21123/bsj.2020.17.3.0760
  • 加载中
图(3) / 表(1)
计量
  • 文章访问数:  853
  • HTML全文浏览量:  140
  • PDF下载量:  42
  • 被引次数: 0
出版历程
  • 收稿日期:  2021-01-13
  • 修回日期:  2021-03-05
  • 网络出版日期:  2021-03-27
  • 刊出日期:  2021-03-31

目录

    /

    返回文章
    返回