Effect of Inoculation Method on Toxicity of Beauveria bassiana against 5th Instar Larvae of Homona coffearia
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摘要: 探讨球孢白僵菌JYBb201和JYBb202菌株在4种染毒方式下对茶卷叶蛾5龄幼虫的毒力,结果表明,当孢子悬液浓度为1.0×107个.mL-1时,球孢白僵菌JYBb201和JYBb202菌株对茶卷叶蛾5龄幼虫的最终校正死亡率分别为:浸虫法85.00%和81.94%;涂抹法82.50%和76.94%;浸梢法47.50%和43.61%;喷雾法52.50%和48.61%。同时,2个菌株采用浸虫法处理茶卷叶蛾5龄幼虫僵虫率也最高,分别为67.50%和57.50%;致死速度最快,LT50分别为3.510d和3.808d。Abstract: Toxicities of Beauveria bassiana strains,JYBb201 and JYBb202,against the 5th instar larvae of Homona coffearia were compared when 4 different methods of inoculation were applied.The adjusted mortality rates of H.coffearia larvae subjected to 1.0×107 conidia·mL-1 spore suspensions of JYBb201 and JYBb202 were found to be 85.00% and 81.94%,respectively,when the larvae were submerged in the spore suspension;82.50% and 76.94%,respectively,when the larvae were smeared with the spore suspension;47.50% and 43.61%,respectively,when the tea tips were dipped in the suspension;and,52.50% and 48.61%,respectively,when the spore suspension was sprayed on the larvae.The immersing method also appeared to bring about the highest cadaver rates at 67.50% for JYBb201 and 57.50% for JYBb202.It was the same when the LT50 values were used for the comparison 3.510 d for JYBb201 and 3.808 d for JYBb202.
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Key words:
- Beauveria bassiana /
- Homona coffearia Meyrick /
- inoculation method /
- toxicity
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